Anti-Onchocerca activity and phytochemical analysis of an essential oil from Cyperus articulatus L

BMC Complementary and Alternative Medicine

Research Article

Moses Samje¹  Fidelis Cho-Ngwa¹  Jonathan Alunge Metuge¹  Kennedy D Nyongbela²  Smith B Babiaka²  James A Mbah²  Ghislain Fotso³ 

[1] ANDI Centre of Excellence, Biotechnology Unit, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon;Department of Chemistry, Faculty of Science, University of Buea, Buea, Cameroon;Department of Organic chemistry, Faculty of Science, University of Yaoundé I, Cameroon;

关键词: Essential oil;    Human onchocerciasis;    Cyperus articulatus;    Phytochemical analysis;   

DOI  :  10.1186/1472-6882-14-223

 received in 2013-12-26, accepted in 2014-06-30,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe lack of a safe and effective adult worm drug and the emergence of resistant animal parasite strains to the only recommended drug, the microfilaricide, ivermectin put many at risk of the devastating effects of the onchocerciasis. The present study was undertaken to investigate the acclaimed anti-Onchocerca activity of the roots/rhizomes of Cyperus articulatus in the traditional treatment of onchocerciasis in North Western Cameroon and to assess the plant as a new source of potential filaricidal lead compounds.MethodsCrude extracts were prepared from the dried plant parts using hexane, methylene chloride and methanol. The antifilarial activity was evaluated in vitro on microfilariae (Mfs) and adult worms of the bovine derived Onchocerca ochengi, a close relative of Onchocerca volvulus. The viabilities of microfilariae and adult male worms were determined based on motility reduction, while for the adult female worms the viability was based on the standard MTT/formazan assay. Cytotoxicity of the active extract was assessed on monkey kidney epithelial cells in vitro and the selectivity indices (SI) were determined. Acute toxicity of the promising extract was investigated in mice. Chemical composition of the active extract was unraveled by GC/MS analysis.ResultsOnly the hexane extract, an essential oil exhibited anti-Onchocerca activity. The oil killed both the microfilariae and adult worms of O. ochengi in a dose manner dependently, with IC50s of 23.4 μg/ml on the Mfs, 23.4 μg/ml on adult male worms and 31.25 μg/ml on the adult female worms. Selectivity indices were 4, 4, and 2.99 for Mfs, adult males and adult females, respectively. At a single limit dose of 2000 mg/kg body weight, none of 6 mice that received the essential oil by gavage died. GC/MS analysis revealed the presence of terpenoids, hydrocarbons and fatty acids or fatty acid derivatives as components of the oil.ConclusionsThe essential oil from the roots/rhizomes of Cyperus articulatus is active against O. ochengi microfilariae and adult worms in vitro in a dose dependent manner, hence may provide a source of new anti-filarial compounds. The results also support the traditional use of C. articulatus in the treatment of human onchocerciasis.

【 授权许可】

Unknown   
© Metuge et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.