Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts

BMC Cancer

Research Article

Hongmei Zhou¹  Qinghong Gao²  Lanyan Wu³  Wenxia Meng⁴  Sixiu Chen⁴  Xin He⁴  Lin Zhang⁴  Qingjie Xia⁵ 

[1] Department of Oral Medicine, West China Hospital of Stomatology, Sichuan University, Sichuan, China;Department of Oral Oncology, West China Hospital of Stomatology, Sichuan University, Sichuan, China;Department of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Sichuan, China;State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China;The Station Key Lab of Biotherapy, Sichuan University, Sichuan, China;

关键词: Oral Squamous Cell Carcinoma;    Oral Squamous Cell Carcinoma;    Oral Squamous Cell Carcinoma Patient;    Oral Epithelium;    Oral Leukoplakia;   

DOI  :  10.1186/1471-2407-11-88

 received in 2010-08-18, accepted in 2011-02-28,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe purpose of this study was to assess the expression levels for TβRI, TβRII, and TβRIII in epithelial layers of oral premalignant lesions (oral leukoplakia, OLK) and oral squamous cell carcinoma (OSCC), as well as in oral carcinoma-associated fibroblasts (CAFs), with the final goal of exploring the roles of various types of TβRs in carcinogenesis of oral mucosa.MethodsNormal oral tissues, OLK, and OSCC were obtained from 138 previously untreated patients. Seven primary human oral CAF lines and six primary normal fibroblast (NF) lines were established successfully via cell culture. The three receptors were detected using immunohistochemical (IHC), quantitative RT-PCR, and Western blot approaches.ResultsIHC signals for TβRII and TβRIII in the epithelial layer decreased in tissue samples with increasing disease aggressiveness (P < 0.05); no expression differences were observed for TβRI, in OLK and OSCC (P > 0.05); and TβRII and TβRIII were significantly downregulated in CAFs compared with NFs, at the mRNA and protein levels (P < 0.05). Exogenous expression of TGF-β1 led to a remarkable decrease in the expression of TβRII and TβRIII in CAFs (P < 0.05).ConclusionThis study provides the first evidence that the loss of TβRII and TβRIII expression in oral epithelium and stroma is a common event in OSCC. The restoration of the expression of TβRII and TβRIII in oral cancerous tissues may represent a novel strategy for the treatment of oral carcinoma.

【 授权许可】

Unknown   
© Meng et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.