Drosophila selenophosphate synthetase 1 regulates vitamin B6 metabolism: prediction and confirmation

BMC Genomics

Research Article

Jin Young Kim¹  Myoung Sup Shim¹  Hee Kyoung Jung¹  Kwang Hee Lee²  Byeong Jae Lee²  Jin Mo Park³  Taesung Park⁴  Eunji Lee⁴  Dolph L Hatfield⁵  Xue-Ming Xu⁵  Bradley A Carlson⁵ 

[1] Department of Biological Sciences, Seoul National University, 151-742, Seoul, Korea;Department of Biological Sciences, Seoul National University, 151-742, Seoul, Korea;Interdisciplinary Program in Bioinformatics, Seoul National University, 151-742, Seoul, Korea;Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, 02114, Boston, MA, USA;Interdisciplinary Program in Bioinformatics, Seoul National University, 151-742, Seoul, Korea;Department of Statistics, Seoul National University, 151-742, Seoul, Korea;Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA;

关键词: Gene Ontology;    Amino Acid Metabolism;    Pyridoxal Phosphate;    Selenophosphate;    Pyridoxine Phosphate;   

DOI  :  10.1186/1471-2164-12-426

 received in 2011-03-21, accepted in 2011-08-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThere are two selenophosphate synthetases (SPSs) in higher eukaryotes, SPS1 and SPS2. Of these two isotypes, only SPS2 catalyzes selenophosphate synthesis. Although SPS1 does not contain selenophosphate synthesis activity, it was found to be essential for cell growth and embryogenesis in Drosophila. The function of SPS1, however, has not been elucidated.ResultsDifferentially expressed genes in Drosophila SL2 cells were identified using two-way analysis of variance methods and clustered according to their temporal expression pattern. Gene ontology analysis was performed against differentially expressed genes and gene ontology terms related to vitamin B6 biosynthesis were found to be significantly affected at the early stage at which megamitochondria were not formed (day 3) after SPS1 knockdown. Interestingly, genes related to defense and amino acid metabolism were affected at a later stage (day 5) following knockdown. Levels of pyridoxal phosphate, an active form of vitamin B6, were decreased by SPS1 knockdown. Treatment of SL2 cells with an inhibitor of pyridoxal phosphate synthesis resulted in both a similar pattern of expression as that found by SPS1 knockdown and the formation of megamitochondria, the major phenotypic change observed by SPS1 knockdown.ConclusionsThese results indicate that SPS1 regulates vitamin B6 synthesis, which in turn impacts various cellular systems such as amino acid metabolism, defense and other important metabolic activities.

【 授权许可】

CC BY   
© Lee et al; licensee BioMed Central Ltd. 2011