Propolis - based chitosan varnish: drug delivery, controlled release and antimicrobial activity against oral pathogen bacteria

BMC Complementary and Alternative Medicine

Research Article

Vagner R Santos¹  Mariana P De Luca²  Allyson N Moreira²  Juçara R Franca³  Tatiana G Ribeiro³  Rachel O Castilho³  André AG Faraco³ 

[1] Department of Oral Clinical, Oral Pathology and Oral Surgery, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;Faculdade de Odontologia UFMG, Campus Pampulha, Av. Pres. Antonio Carlos, 6627, 31270-901, Pampulha, Belo Horizonte, MG, Brazil;Department of Restorative Dentistry, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;Pharmaceutical Science Post-Graduation Program, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;

关键词: Propolis;    Chitosan;    Varnish;    Drug delivery;    Cariogenic biofilm;    Prevention;    Oral microorganisms;   

DOI  :  10.1186/1472-6882-14-478

 received in 2014-02-05, accepted in 2014-10-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDental caries is the most prevalent oral disease in several Asian and Latin American countries. It is an infectious disease and different types of bacteria are involved in the process. Synthetic antimicrobials are used against this disease; however, many of these substances cause unwarranted undesirable effects like vomiting, diarrhea and tooth staining. Propolis, a resinous substance collected by honeybees, has been used to control the oral microbiota. So, the objective of this study was to develop and characterize sustained-release propolis-based chitosan varnish useful on dental cariogenic biofilm prevention, besides the in vitro antimicrobial activity.MethodsThree formulations of propolis - based chitosan varnish (PCV) containing different concentrations (5%, 10% and 15%) were produced by dissolution of propolis with chitosan on hydro-alcoholic vehicle. Bovine teeth were used for testing adhesion of coatings and to observe the controlled release of propolis associated with varnish. It was characterized by infrared spectroscopy, scanning electron microscopy, casting time, diffusion test in vitro antimicrobial activity and controlled release. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested for the main microorganisms involved in the cariogenic biofilm through the microdilution test in 96-well plates.ResultsThe formulations presented a tooth surface adherence and were able to form films very fast on bovine tooth surface. Also, propolis-based chitosan varnishes have shown antimicrobial activity similar to or better than chlorhexidine varnish against all oral pathogen bacteria. All microorganisms were sensitive to propolis varnish and chitosan. MIC and MBC for microorganisms of cariogenic biofilme showed better results than chlorhexidine. Propolis active components were released for more than one week.ConclusionAll developed formulations turn them, 5%, 10% and 15% propolis content varnish, into products suitable for clinical application on dental caries prevention field, deserving clinical studies to confirm its in vivo activity.

【 授权许可】

Unknown   
© Franca et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.