Antigenotoxic properties of Paliurus spina-christi Mill fruits and their active compounds

BMC Complementary and Alternative Medicine

Research Article

Nurşen Başaran¹  Sevtap Aydin¹  Murat Zor²  Arif Ahmet Başaran³  Nadide Deniz Güner⁴ 

[1] Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey;Department of Pharmacognosy, Faculty of Pharmacy, Bülent Ecevit University, 67100, Zonguldak, Turkey;Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey;Republic of Turkey Social Security Institution, 06520, Ankara, Turkey;

关键词: Paliurus spina-christi;    Active compounds;    Homo- and hetero-NMR;    MTT assay;    Comet assay;    Chinese hamster lung fibroblast cells;   

DOI  :  10.1186/s12906-017-1732-1

 received in 2016-09-22, accepted in 2017-04-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPaliurus spina-christi Mill. (PS) fruits are widely used for different medical purposes in Turkey. Like in many medicinal herbs the studies concerning their activity, the activities of PS are also not well clarified. The aim of this study is to evaluate the antigenotoxicity of the compounds isolated and identified from the extracts of PS fruits.MethodsThe active compounds were separated, isolated, and determined by chromatographic methods and their structural elucidation was performed by Nuclear Magnetic Resonance (NMR) methods. The compounds were obtained from either ethyl acetate (EA) or n-butanol extracts. The cytotoxicities of the compounds using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the antigenotoxic activities of the compounds using the alkaline single cell gel electrophoresis techniques (comet assay) were evaluated in Chinese hamster lung fibroblast (V79) cell lines.ResultsThe isolated major compounds were identified as (+/−) catechins and gallocatechin from EA fraction and rutin from n-butanol fraction of PS fruits. Their chemical structures were identified by 1H-NMR, 13C-NMR, HMBC, and HMQC techniques. Half-maximal inhibitory concentration of catechins, gallocatechin, and rutin were found to be 734 μg/mL, 220 μg/mL, and 1004 μg/mL, respectively. The methanolic extract of PS (1-100 μg/mL) alone did not induce DNA single-strand breaks while catechins (1-100 μg/mL), gallocatechin (1-50 μg/mL), and rutin (1-50 μg/mL) significantly reduced H2O2-induced DNA damage.ConclusionIt has been suggested that PS fruits and their compounds catechins, gallocatechin and rutin may have beneficial effects in oxidative DNA damage. It seems that PS fruits may be used in protection of the disorders related to DNA damage.

【 授权许可】

CC BY   
© The Author(s). 2017