Mineralising and antibacterial effects of modified calcium phosphate treatment on human root cementum

BMC Oral Health

Research Article

Jin-Long Gao¹  Xiaoyan Zhou¹  Ziming Zhao²  Limin Liu³  Junqi Ling³  Haijing Gu⁴ 

[1] Faculty of Dentistry, University of Sydney, Sydney, NSW, Australia;Institute of Dental Research, Westmead Centre for Oral Health, Westmead Millennium Institute, 2153, Westmead, NSW, Australia;Guangdong Provincial Institute of Traditional Chinese Medicine, Guangzhou, China;Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, 56 Ling Yuan Xi Road, 510055, Guangzhou, China;Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, 56 Ling Yuan Xi Road, 510055, Guangzhou, China;Department of Biomaterials & Biomimetics, New York University College of Dentistry, New York City, USA;

关键词: Root caries;    Cementum;    Modified calcium phosphate;    Remineralisation;    Anti-microbial;   

DOI  :  10.1186/s12903-016-0246-4

 received in 2015-07-16, accepted in 2016-07-06,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAging population will lead to the increase of incidence of root caries globally. The clinical management of root caries is challenging due to the difficulty in moisture isolation. The root caries is caused by the release of organic acids from cariogenic bacteria which results in the dissolution of cementum and dentin of the root. The purpose of this study is to study the efficacy of modified saturated calcium phosphate solution (CaP) supplement with zinc (Zn2+) and/or fluoride (F-) in providing root cementum surfaces less susceptible to acid dissolution and bacterial colonization.MethodsHuman root cementum sections from extracted premolars were treated with three modified calcium phosphate solutions (M/A-CaPs) respectively: (A) CaP-F/Zn, supplemented with F- and Zn2+; (B) CaP-F, supplemented with F- only; (C) CaP-Zn, supplemented with Zn2+ only. The surface characteristics of treated cementum sections were investigated using scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FT-IR). Following the acid attack and Streptococcus mutans challenge, M/A-CaPs treated cementum surfaces were analysed using inductive coupled plasma (ICP) and SEM respectively.ResultsCompared with the control group, M/A-CaPs treated cementum presented significant improvements in resistance to acid dissolution and bacterial colonization. Among M/A-CaPs, the CaP-F/Zn treated cementum surfaces released the lowest amount of Ca2+ ions (2.11 ± 0.51 ppm) upon acid challenge (n = 3, p < 0.01) and also presented the most significant inhibiting effect against the colonization of S. mutans (n = 180, p < 0.05).ConclusionsSaturated calcium phosphate solution CaP supplemented with both F- and Zn2+ could be applied as an effective coating material providing acid resistance and antibacterial property on cementum surfaces. The modified calcium phosphate-based solution could be a new treatment strategy to prevent the development of root caries and arrest the further progression of root caries.

【 授权许可】

CC BY   
© The Author(s). 2016