Increase in the astaxanthin synthase gene (crtS) dose by in vivo DNA fragment assembly in Xanthophyllomyces dendrorhous

BMC Biotechnology

Research Article

Víctor Cifuentes¹  Marcelo Baeza¹  Jennifer Alcaíno¹  Gabriela Contreras¹  Salvador Barahona¹  María Cecilia Rojas² 

[1] Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, 653, Casilla, Santiago, Chile;Departamento de Química, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, 653, Casilla, Santiago, Chile;

关键词: Xanthophyllomyces dendrorhous;    Astaxanthin synthase;    DNA assembler;   

DOI  :  10.1186/1472-6750-13-84

 received in 2013-07-12, accepted in 2013-10-04,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundXanthophyllomyces dendrorhous is a basidiomycetous yeast that is relevant to biotechnology, as it can synthesize the carotenoid astaxanthin. However, the astaxanthin levels produced by wild-type strains are low. Although different approaches for promoting increased astaxanthin production have been attempted, no commercially competitive results have been obtained thus far. A promising alternative to facilitate the production of carotenoids in this yeast involves the use of genetic modification. However, a major limitation is the few available molecular tools to manipulate X. dendrorhous.ResultsIn this work, the DNA assembler methodology that was previously described in Saccharomyces cerevisiae was successfully applied to assemble DNA fragments in vivo and integrate these fragments into the genome of X. dendrorhous by homologous recombination in only one transformation event. Using this method, the gene encoding astaxanthin synthase (crtS) was overexpressed in X. dendrorhous and a higher level of astaxanthin was produced.ConclusionsThis methodology could be used to easily and rapidly overexpress individual genes or combinations of genes simultaneously in X. dendrorhous, eliminating numerous steps involved in conventional cloning methods.

【 授权许可】

Unknown   
© Contreras et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.