The distinctive cell division interactome of Neisseria gonorrhoeae

BMC Microbiology

Research Article

Yinan Zou¹  Jo-Anne R. Dillon²  Yan Li³ 

[1] Department of Microbiology and Immunology, College of Medicine, S7N 5E5, Saskatoon, SK, Canada;Vaccine and Infectious Disease Organization, International Vaccine Centre, S7N 5E3, Saskatoon, SK, Canada;Department of Microbiology and Immunology, College of Medicine, S7N 5E5, Saskatoon, SK, Canada;Vaccine and Infectious Disease Organization, International Vaccine Centre, S7N 5E3, Saskatoon, SK, Canada;Department of Biology, College of Arts and Science, University of Saskatchewan, S7N 5A5, Saskatoon, SK, Canada;Vaccine and Infectious Disease Organization, International Vaccine Centre, S7N 5E3, Saskatoon, SK, Canada;Department of Biology, College of Arts and Science, University of Saskatchewan, S7N 5A5, Saskatoon, SK, Canada;

关键词: Cell division;    Interactome;    N. gonorrhoeae;    Protein-protein interaction;    Bacterial two-hybrid assay;    Surface plasmon resonance;    GST pull-down;    FtsA domains;   

DOI  :  10.1186/s12866-017-1140-1

 received in 2017-06-12, accepted in 2017-12-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBacterial cell division is an essential process driven by the formation of a Z-ring structure, as a cytoskeletal scaffold at the mid-cell, followed by the recruitment of various proteins which form the divisome. The cell division interactome reflects the complement of different interactions between all divisome proteins. To date, only two cell division interactomes have been characterized, in Escherichia coli and in Streptococcus pneumoniae. The cell divison proteins encoded by Neisseria gonorrhoeae include FtsZ, FtsA, ZipA, FtsK, FtsQ, FtsI, FtsW, and FtsN. The purpose of the present study was to characterize the cell division interactome of N. gonorrhoeae using several different methods to identify protein-protein interactions. We also characterized the specific subdomains of FtsA implicated in interactions with FtsZ, FtsQ, FtsN and FtsW.ResultsUsing a combination of bacterial two-hybrid (B2H), glutathione S-transferase (GST) pull-down assays, and surface plasmon resonance (SPR), nine interactions were observed among the eight gonococcal cell division proteins tested. ZipA did not interact with any other cell division proteins. Comparisons of the N. gonorrhoeae cell division interactome with the published interactomes from E. coli and S. pneumoniae indicated that FtsA-FtsZ and FtsZ-FtsK interactions were common to all three species. FtsA-FtsW and FtsK-FtsN interactions were only present in N. gonorrhoeae. The 2A and 2B subdomains of FtsANg were involved in interactions with FtsQ, FtsZ, and FtsN, and the 2A subdomain was involved in interaction with FtsW.ConclusionsResults from this research indicate that N. gonorrhoeae has a distinctive cell division interactome as compared with other microorganisms.

【 授权许可】

CC BY   
© The Author(s). 2017