Expression and function of phosphodiesterases (PDEs) in the rat urinary bladder

BMC Urology

Research Article

Xiaofei Zhu¹  Yue Mi²  Guangju Ji³  Kui Zhai³ 

[1] Department of Urology, Beijing Jishuitan Hospital, Beijing, China;Department of Urology, Peking University First Hospital, Beijing, China;National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, 100101, Beijing, China;

关键词: Gene expression;    Phasic contraction;    Phosphodiesterases;    Rat urinary bladder;   

DOI  :  10.1186/s12894-017-0244-0

 received in 2016-07-20, accepted in 2017-06-29,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIt has been shown that hosphodiesterases (PDEs) play an important role in mediating the smooth muscle tone of rat urinary bladder. However, the gene expression profiles of them were still unknown.MethodsUrinary bladder Strips were obtained from both neonatal and adult Sprague-Dawley rats. RT-PCR/western blot and organ bath were used to measure the expression and function of PDEs.ResultsAdult rat urinary bladder expressed various PDE mRNA with the following rank order: PDE5A ≈ PDE9A ≈ PDE10A > PDE2A ≈ PDE4A ≈ PDE4D > PDE4B ≈ PDE3B ≈ PDE8B ≈ PDE7A ≈ PDE7B > PDE1A. PDE1B, PDE1C, PDE3A, PDE4C, PDE8A, and PDE11A were not detected. Of interest, the mRNA and protein of PDE3A were significantly decreased in adult rat urinary bladder compared to neonatal rat urinary bladder. Cilostamide, a specific inhibitor for PDE3, significantly inhibited the amplitude and frequency of carbachol-enhanced phasic contractions of neonatal rat bladder strips by 38.8% and 12.1%, respectively. Compared to the neonatal rat bladder, the effect of cilostamide was significantly blunted in adult rat urinary bladder: the amplitude and frequency of carbachol-enhanced phasic contractions were decreased by 13.4% (P < 0.01 vs neonatal rat bladder) and 4.4%, respectively. However, the mRNA and the protein levels of PDE3B were similar between neonatal and adult rat bladder.ConclusionWe found that several PDE isoforms were expressed in the rat urinary bladder with distinct levels. Moreover, we showed that the function of PDE3 was blunted in adult rat bladder likely due to the decreased expression of PDE3A.

【 授权许可】

CC BY   
© The Author(s). 2017