A genome scan for quantitative trait loci affecting cyanogenic potential of cassava root in an outbred population

BMC Genomics

Research Article

Supannee Poopear¹  Supanath Kanjanawattanawong¹  Sukhuman Whankaew¹  Kanokporn Triwitayakorn²  Sithichoke Tangphatsornruang³  David A Lightfoot⁴  Opas Boonseng⁵ 

[1] Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand;Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand;Center for Cassava Molecular Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand;National Center for Genetic Engineering and Biotechnology, Bangkok, Thailand;Center for Cassava Molecular Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand;Plant Biotechnology and Genomics Core-Facility, Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, 62901, Carbondale, IL, USA;Rayong Filed Crops Research Center, Ministry of Agriculture and Cooperatives, Rayong, Thailand;

关键词: Quantitative Trait Locus;    Linkage Group;    Quantitative Trait Locus Analysis;    Cyanogenic Glycoside;    Linamarin;   

DOI  :  10.1186/1471-2164-12-266

 received in 2010-06-10, accepted in 2011-05-25,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCassava (Manihot esculenta Crantz) can produce cyanide, a toxic compound, without self-injury. That ability was called the cyanogenic potential (CN). This project aimed to identify quantitative trait loci (QTL) associated with the CN in an outbred population derived from 'Hanatee' × 'Huay Bong 60', two contrasting cultivars. CN was evaluated in 2008 and in 2009 at Rayong province, and in 2009 at Lop Buri province, Thailand. CN was measured using a picrate paper kit. QTL analysis affecting CN was performed with 303 SSR markers.ResultsThe phenotypic values showed continuous variation with transgressive segregation events with more (115 ppm) and less CN (15 ppm) than either parent ('Hanatee' had 33 ppm and 'Huay Bong 60' had 95 ppm). The linkage map consisted of 303 SSR markers, on 27 linkage groups with a map that encompassed 1,328 cM. The average marker interval was 5.8 cM. Five QTL underlying CN were detected. CN08R1 from 2008 at Rayong, CN09R1 and CN09R2 from 2009 at Rayong, and CN09L1 and CN09L2 from 2009 at Lop Buri were mapped on linkage group 2, 5, 10 and 11, respectively. Among all the identified QTL, CN09R1 was the most significantly associated with the CN trait with LOD score 5.75 and explained the greatest percentage of phenotypic variation (%Expl.) of 26%.ConclusionsFive new QTL affecting CN were successfully identified from 4 linkage groups. Discovery of these QTL can provide useful markers to assist in cassava breeding and studying genes affecting the trait.

【 授权许可】

Unknown   
© Whankaew et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.