Hominis Placenta facilitates hair re-growth by upregulating cellular proliferation and expression of fibroblast growth factor-7

BMC Complementary and Alternative Medicine

Research Article

Jae-ho Chung¹  Ha Rim Kim²  Chang-Hyun Lee³  Dong-Jin Lee⁴  Hyung-Sik Seo⁵  Jae Eun Kim⁶  Han-Sol Jeong⁷  Ki-Tae Ha⁷  Byung Joo Kim⁸  Myeong Ho Jung⁸ 

[1] Chung Jaeho Traditional Korean Medical Clinic, Seoul, Republic of Korea;College of food & biotechnology, Woosuk University, Wanju, Republic of Korea;Department of Anatomy, College of Korean Medicine, Woosuk University, Wanju, Republic of Korea;Department of Clinical Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea;Department of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University, Yangsan, Republic of Korea;Department of Pathology, College of Korean Medicine, Dongguk University, Goyang, Republic of Korea;Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea;Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea;Healthy Aging Korean Medical Research Center, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea;

关键词: Hominis Placenta;    Hair growth;    Anagen;    Bromodeoxyuridine;    Fibroblast growth factor-7;   

DOI  :  10.1186/s12906-016-1180-3

 received in 2015-07-07, accepted in 2016-06-15,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHominis Placenta (HP) known as a restorative medicine in Traditional Chinese Medicine (TCM), has been widely applied in the clinics of Korea and China as an anti-aging agent to enhance the regeneration of tissue. This study was conducted to investigate whether topical treatment of HP promotes hair regrowth in the animal model.MethodsThe dorsal hairs of 8-week-old C57BL/6 mice were depilated to synchronize hair follicles to the anagen phase. HP was applied topically once a day for 15 days. Hair growth was evaluated visually and microscopically. The incorporation of bromodeoxyuridine (BrdU) and expression of proliferating cell nuclear antigen (PCNA), fibroblast growth factor-7 (FGF-7) in dorsal skin tissue was examined by immunohistochemical analysis. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the mRNA expression of FGF-7.ResultsHP exhibited potent hair growth-promoting activity in C57BL/6 mice. Gross examination indicated that HP markedly increased hair regrowth as well as hair density and diameter. Histologic analysis showed that HP treatment enhanced the anagen induction of hair follicles.Immunohistochemical analysis revealed that BrdU incorporation and the expressions of PCNA were increased by treatment of HP. HP treatment significantly increased the expression of FGF-7, which plays pivotal roles to maintain anagen phase both protein and mRNA levels.ConclusionsTaken together, our results indicate that HP has a potent hair growth-promoting activity; therefore, it may be a good candidate for the treatment of alopecia.

【 授权许可】

CC BY   
© The Author(s). 2016