Global transcriptome analysis reveals distinct expression among duplicated genes during sorghum-Bipolaris sorghicolainteraction

BMC Plant Biology

Research Article

Hiroshi Minami¹  Yoshihiro Kawahara²  Hiroshi Mizuno²  Hiroyuki Kanamori²  Takeshi Itoh²  Takashi Matsumoto²  Hiroyuki Kawahigashi²  Jun Ogata² 

[1] Mitsubishi Space Software Co. Ltd, Takezono 1-6-1, 305-0032, Tsukuba, Ibaraki, Japan;National Institute of Agrobiological Sciences (NIAS), Agrogenomics Research Center, 1-2, Kannondai 2-chome, 305-8602, Tsukuba, Ibaraki, Japan;

关键词: Naringenin;    Isocitrate Lyase;    Glyoxylate Shunt;    Isoquinoline Alkaloid;    Secondary Metabolic Pathway;   

DOI  :  10.1186/1471-2229-12-121

 received in 2011-10-09, accepted in 2012-07-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundSorghum (Sorghum bicolor L. Moench) is a rich source of natural phytochemicals. We performed massive parallel sequencing of mRNA to identify differentially expressed genes after sorghum BTx623 had been infected with Bipolaris sorghicola, a necrotrophic fungus causing a sorghum disease called target leaf spot.ResultSeventy-six-base-pair reads from mRNAs of mock- or pathogen-infected leaves were sequenced. Unannotated transcripts were predicted on the basis of the piling-up of mapped short reads. Differentially expressed genes were identified statistically; particular genes in tandemly duplicated putative paralogs were highly upregulated. Pathogen infection activated the glyoxylate shunt in the TCA cycle; this changes the role of the TCA cycle from energy production to synthesis of cell components. The secondary metabolic pathways of phytoalexin synthesis and of sulfur-dependent detoxification were activated by upregulation of the genes encoding amino acid metabolizing enzymes located at the branch point between primary and secondary metabolism. Coordinated gene expression could guide the metabolic pathway for accumulation of the sorghum-specific phytochemicals 3-deoxyanthocyanidin and dhurrin. Key enzymes for synthesizing these sorghum-specific phytochemicals were not found in the corresponding region of the rice genome.ConclusionPathogen infection dramatically changed the expression of particular paralogs that putatively encode enzymes involved in the sorghum-specific metabolic network.

【 授权许可】

Unknown   
© Mizuno et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.