Expiratory flow rate, breath hold and anatomic dead space influence electronic nose ability to detect lung cancer

BMC Pulmonary Medicine

Research Article

Marton Hernadi¹  Beata Zita Korosi²  Ildiko Horvath²  Gyorgy Losonczy²  Zoltan Sutto²  Gabriella Zsamboki²  Laszlo Kunos²  Andras Bikov²  David Laszlo Tarnoki³  Adam Domonkos Tarnoki³ 

[1] Department of Pediatrics, Heim Pal Children’s Hospital, 86 Ulloi street, 1082, Budapest, Hungary;Department of Pulmonology, Semmelweis University, 1/C Dios arok, 1125, Budapest, Hungary;Department of Radiology and Oncotherapy, Semmelweis University, 78/a Ulloi street, 1082, Budapest, Hungary;

关键词: Biomarkers;    Breath test;    Electronic nose;    Lung cancer;    Volatile organic compounds;   

DOI  :  10.1186/1471-2466-14-202

 received in 2013-10-12, accepted in 2014-12-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundElectronic noses are composites of nanosensor arrays. Numerous studies showed their potential to detect lung cancer from breath samples by analysing exhaled volatile compound pattern (“breathprint”). Expiratory flow rate, breath hold and inclusion of anatomic dead space may influence the exhaled levels of some volatile compounds; however it has not been fully addressed how these factors affect electronic nose data. Therefore, the aim of the study was to investigate these effects.Methods37 healthy subjects (44 ± 14 years) and 27 patients with lung cancer (60 ± 10 years) participated in the study. After deep inhalation through a volatile organic compound filter, subjects exhaled at two different flow rates (50 ml/sec and 75 ml/sec) into Teflon-coated bags. The effect of breath hold was analysed after 10 seconds of deep inhalation. We also studied the effect of anatomic dead space by excluding this fraction and comparing alveolar air to mixed (alveolar + anatomic dead space) air samples. Exhaled air samples were processed with Cyranose 320 electronic nose.ResultsExpiratory flow rate, breath hold and the inclusion of anatomic dead space significantly altered “breathprints” in healthy individuals (p < 0.05), but not in lung cancer (p > 0.05). These factors also influenced the discrimination ability of the electronic nose to detect lung cancer significantly.ConclusionsWe have shown that expiratory flow, breath hold and dead space influence exhaled volatile compound pattern assessed with electronic nose. These findings suggest critical methodological recommendations to standardise sample collections for electronic nose measurements.

【 授权许可】

CC BY   
© Bikov et al.; licensee BioMed Central. 2014