Calcitonin substitution in calcitonin deficiency reduces particle-induced osteolysis

BMC Musculoskeletal Disorders

Research Article

Gero Hilken¹  Martina Broecker-Preuss²  Christian Wedemeyer³  Laura Neuerburg³  Marius von Knoch⁴  Max D Kauther⁵  Florian Grabellus⁶  Gabriele Koehler⁷  Hagen S Bachmann⁸ 

[1]Central Animal Laboratory, University of Duisburg-Essen, Hufelandstr. 55, 45122, Essen, Germany

[2]Department of Endocrinology, Division of Clinical Chemistry and Laboratory Medicine, University Duisburg-Essen, Hufelandstr. 55, 45122, Essen, Germany

[3]Department of Orthopaedics, University Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany

[4]Department of Orthopaedics, University Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany

[5]Department of Orthopaedic Surgery, Klinikum Bremerhaven, Postbrookstr. 103, 27574, Bremerhaven, Germany

[6]Department of Trauma Surgery, University Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany

[7]Department of Orthopaedics, University Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany

[8]Institute of Pathology and Neuropathology, University of Duisburg-Essen, Hufelandstraße 55, 45122, Essen, Germany

[9]Institute of Pathology, University of Münster, Domagkstraße 5, 48149Münster, Germany

[10]Institute of Pharmacogenetics, University Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany

关键词: Bone Resorption;    Calcitonin;    UHMWPE;    Aseptic Loosening;    Bone Thickness;   

DOI  :  10.1186/1471-2474-12-186

 received in 2011-03-08, accepted in 2011-08-15,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundPeriprosthetic osteolysis is a major cause of aseptic loosening in joint arthroplasty. This study investigates the impact of CT (calcitonin) deficiency and CT substitution under in-vivo circumstances on particle-induced osteolysis in Calca -/- mice.MethodsWe used the murine calvarial osteolysis model based on ultra-high molecular weight polyethylene (UHMWPE) particles in 10 C57BL/6J wild-type (WT) mice and twenty Calca -/- mice. The mice were divided into six groups: WT without UHMWPE particles (Group 1), WT with UHMWPE particles (Group 2), Calca -/- mice without UHMWPE particles (Group 3), Calca -/- mice with UHMWPE particles (Group 4), Calca -/- mice without UHMWPE particles and calcitonin substitution (Group 5), and Calca -/- mice with UHMWPE particle implantation and calcitonin substitution (Group 6). Analytes were extracted from serum and urine. Bone resorption was measured by bone histomorphometry. The number of osteoclasts was determined by counting the tartrate-resistant acid phosphatase (TRACP) + cells.ResultsBone resorption was significantly increased in Calca -/- mice compared with their corresponding WT. The eroded surface in Calca -/- mice with particle implantation was reduced by 20.6% after CT substitution. Osteoclast numbers were significantly increased in Calca -/- mice after particle implantation. Serum OPG (osteoprotegerin) increased significantly after CT substitution.ConclusionsAs anticipated, Calca -/- mice show extensive osteolysis compared with wild-type mice, and CT substitution reduces particle-induced osteolysis.

【 授权许可】

CC BY   
© Kauther et al; licensee BioMed Central Ltd. 2011