A streamlined tethered chromosome conformation capture protocol

BMC Genomics

Methodology Article

Idan Gabdank¹  Anne M. Villeneuve²  Sreejith Ramakrishnan²  Andrew Z. Fire³ 

[1] Department of Genetics, Stanford University School of Medicine, 94304, Stanford, CA, USA;Departments of Developmental Biology and Genetics, Stanford University School of Medicine, 94304, Stanford, California, USA;Departments of Pathology and Genetics, Stanford University School of Medicine, 94304, Stanford, California, USA;

关键词: Hi-C;    TCC;    Chromatin;    Conformation;    Caenorhabditis elegans;    Genome;   

DOI  :  10.1186/s12864-016-2596-3

 received in 2015-10-14, accepted in 2016-03-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundIdentification of locus-locus contacts at the chromatin level provides a valuable foundation for understanding of nuclear architecture and function and a valuable tool for inferring long-range linkage relationships. As one approach to this, chromatin conformation capture-based techniques allow creation of genome spatial organization maps. While such approaches have been available for some time, methodological advances will be of considerable use in minimizing both time and input material required for successful application.ResultsHere we report a modified tethered conformation capture protocol that utilizes a series of rapid and efficient molecular manipulations. We applied the method to Caenorhabditis elegans, obtaining chromatin interaction maps that provide a sequence-anchored delineation of salient aspects of Caenorhabditis elegans chromosome structure, demonstrating a high level of consistency in overall chromosome organization between biological samples collected under different conditions. In addition to the application of the method to defining nuclear architecture, we found the resulting chromatin interaction maps to be of sufficient resolution and sensitivity to enable detection of large-scale structural variants such as inversions or translocations.ConclusionOur streamlined protocol provides an accelerated, robust, and broadly applicable means of generating chromatin spatial organization maps and detecting genome rearrangements without a need for cellular or chromatin fractionation.

【 授权许可】

CC BY   
© Gabdank et al. 2016