期刊论文详细信息
BMC Complementary and Alternative Medicine
Flavones from Erythrina falcata are modulators of fear memory
Research Article
Cláudia R Zamberlam1  Renan Barreta Gaiardo1  Suzete M Cerutti1  Daniela Rodrigues de Oliveira2  Gizelda Maia Rêgo3  Janete M Cerutti4  Alberto J Cavalheiro5 
[1] Department of Biological Science, Behavior Pharmacology and Ethnopharmacology Laboratory, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil;Department of Biological Science, Behavior Pharmacology and Ethnopharmacology Laboratory, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil;Department of Morphology and Genetics, Genetic Bases of Thyroid Tumor Laboratory, Division of Genetics, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil;Department of Forestry Colombo, Brazilian Agricultural Research Corporation (EMBRAPA), Paraná, Brazil;Department of Morphology and Genetics, Genetic Bases of Thyroid Tumor Laboratory, Division of Genetics, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil;Institute of Chemistry, Nuclei of Bioassay, Biosynthesis and Ecophysiology of Natural Products (NuBBE), State University of Sao Paulo, UNESP, Araraquara, SP, Brazil;
关键词: Flavone;    Acquisition;    Extinction;    Fear conditioning;    Erythrina falcata;    HPLC-ESI/MS;   
DOI  :  10.1186/1472-6882-14-288
 received in 2013-10-01, accepted in 2014-07-24,  发布年份 2014
来源: Springer
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【 摘 要 】

BackgroundFlavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. The aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory.MethodsThe secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). The buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 – BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. The BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. The isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). The activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats.ResultsSix subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovitexin and 6-C-glycoside-diosmetin improved the acquisition of fear memory. Rats treated with BuF, BuF3 and BuF4 were particularly resistant to extinction. Nevertheless, rats treated with FfA and FfB, vitexin, isovitexin and 6-C-glycoside-diosmetin exhibited gradual reduction in conditioned fear response during the extinction retest session, which was measured at 48 to 480 h after conditioning.ConclusionsOur results demonstrate that vitexin, isovitexin and diosmetin-6-C-glucoside and flavonoidic fractions resulted in a significant retention of fear memory but did not prevent the extinction of fear memory. These results further substantiate that the treatment with pure flavonoids or flavanoid-rich fractions might represent potential therapeutic approaches for the treatment of neurocognitive disorders, improvement of memory acquisition and spontaneous recovery of fear.

【 授权许可】

Unknown   
© de Oliveira et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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