Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

BMC Genomics

Research Article

Cathy Coutu¹  Stephen J. Robinson¹  M. Hossein Borhan¹  Shirin Seifbarghi²  Dwayne D. Hegedus³  Yangdou Wei⁴ 

[1] Agriculture and Agri-Food Canada, 107 Science Place, S7N 0X2, Saskatoon, SK, Canada;Agriculture and Agri-Food Canada, 107 Science Place, S7N 0X2, Saskatoon, SK, Canada;Department of Biology, University of Saskatchewan, Saskatoon, Canada;Agriculture and Agri-Food Canada, 107 Science Place, S7N 0X2, Saskatoon, SK, Canada;Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Canada;Department of Biology, University of Saskatchewan, Saskatoon, Canada;

关键词: Sclerotinia sclerotiorum;    Brassica napus;    Infection;    Transcriptome;    Necrosis;    Effectors;    Hydrolytic enzymes;    Secondary metabolites;    Oxalic acid;   

DOI  :  10.1186/s12864-017-3642-5

 received in 2016-10-14, accepted in 2017-03-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSclerotinia sclerotiorum causes stem rot in Brassica napus, which leads to lodging and severe yield losses. Although recent studies have explored significant progress in the characterization of individual S. sclerotiorum pathogenicity factors, a gap exists in profiling gene expression throughout the course of S. sclerotiorum infection on a host plant. In this study, RNA-Seq analysis was performed with focus on the events occurring through the early (1 h) to the middle (48 h) stages of infection.ResultsTranscript analysis revealed the temporal pattern and amplitude of the deployment of genes associated with aspects of pathogenicity or virulence during the course of S. sclerotiorum infection on Brassica napus. These genes were categorized into eight functional groups: hydrolytic enzymes, secondary metabolites, detoxification, signaling, development, secreted effectors, oxalic acid and reactive oxygen species production. The induction patterns of nearly all of these genes agreed with their predicted functions. Principal component analysis delineated gene expression patterns that signified transitions between pathogenic phases, namely host penetration, ramification and necrotic stages, and provided evidence for the occurrence of a brief biotrophic phase soon after host penetration.ConclusionsThe current observations support the notion that S. sclerotiorum deploys an array of factors and complex strategies to facilitate host colonization and mitigate host defenses. This investigation provides a broad overview of the sequential expression of virulence/pathogenicity-associated genes during infection of B. napus by S. sclerotiorum and provides information for further characterization of genes involved in the S. sclerotiorum-host plant interactions.

【 授权许可】

CC BY   
© The Author(s). 2017