Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields

BMC Genomics

Research Article

Christophe Lelong¹  Aude Jouaux¹  Maxime Lafont¹  Michel Mathieu¹  Maryline Houssin²  Jean-Louis Blin³ 

[1] CNRS INEE, BioMEA, 14 032, Caen Cedex, France;Biologie des Organismes Marins et des Ecosystèmes Associés (BioMEA), IBFA, SFR ICORE, Université de Caen Basse-Normandie, 14032, Caen Cedex, France;Centre de Référence sur l’Huître, Université de Caen Basse Normandie, 14 032, Caen Cedex, France;Centre de Référence sur l’Huître, Université de Caen Basse Normandie, 14 032, Caen Cedex, France;Laboratoire Frank Duncombe, 14 053, Saint Contest Cedex 4, France;Centre de Référence sur l’Huître, Université de Caen Basse Normandie, 14 032, Caen Cedex, France;Synergie Mer Et Littoral, Zone conchylicole, 50 560, Blainville sur mer, France;

关键词: Crassostrea gigas;    OsHV-1;    Host response;    Mortality;    Transcriptome;   

DOI  :  10.1186/1471-2164-14-590

 received in 2013-03-01, accepted in 2013-08-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundMassive mortalities have been observed in France since 2008 on spat and juvenile Pacific oysters, Crassostrea gigas. A herpes virus called OsHV-1, easily detectable by PCR, has been implicated in the mortalities as demonstrated by the results of numerous field studies linking mortality with OsHV-1 prevalence. Moreover, experimental infections using viral particles have documented the pathogenicity of OsHV-1 but the physiological responses of host to pathogen are not well known.ResultsThe aim of this study was to understand mechanisms brought into play against the virus during infection in the field. A microarray assay has been developed for a major part of the oyster genome and used for studying the host transcriptome across mortality on field. Spat with and without detectable OsHV-1 infection presenting or not mortality respectively were compared by microarray during mortality episodes. In this study, a number of genes are regulated in the response to pathogen infection on field and seems to argue to an implication of the virus in the observed mortality. The result allowed establishment of a hypothetic scheme of the host cell’s infection by, and response to, the pathogen.ConclusionsThis response shows a “sensu stricto” innate immunity through genic regulation of the virus OsHV-1 life cycle, but also others biological processes resulting to complex interactions between host and pathogens in general.

【 授权许可】

CC BY   
© Jouaux et al.; licensee BioMed Central Ltd. 2013