BMC Cancer | |
miR-302b inhibits tumorigenesis by targeting EphA2 via Wnt/ β-catenin/EMT signaling cascade in gastric cancer | |
Research Article | |
Jian Ma1  Howard L. Mcleod2  Yijing He3  Jie Ge4  Zihua Chen4  Pan Chen5  Shan Zeng6  Lanhua Tang6  Jin Huang6  Li Li6  Liangfang Shen6  Yanchun Xie6  Desheng Xiao7  Xianli Yin8  Huabin Hu9  | |
[1] Cancer Research Institute, Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Central South University, No.138 Tongzipo Road, Changsha, China;Department of Clinical Pharmacology, XiangYa Hospital, Central South University, 410008, Changsha, China;Hunan Key Laboratory of Pharmacogenetics, 410008, Changsha, China;Moffitt Cancer Center, DeBartolo Family Personalized Medicine Institute, 33612, Tampa, FL, USA;Department of Dermatology, XiangYa Hospital, Central South University, 410008, Changsha, China;Department of General Surgery, Xiangya Hospital of Central South University, No.87 Xiangya Road, 410008, Changsha, People’s Republic of China;Department of Hepatobiliary Surgery, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 410013, Changsha, China;Department of Oncology, Xiangya Hospital, Central South University, 410008, Changsha, China;Department of Pathology, Xiangya Hospital, Central South University, 410008, Changsha, China;Department of gastroenterology and urology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 410013, Changsha, China;The Sixth Affiliated Hospital of Sun Yat-Sen University, 510655, Guangzhou, China; | |
关键词: miR-302b; EphA2; Gastric cancer; Epithelial-mesenchymal transition; Wnt/β-catenin; | |
DOI : 10.1186/s12885-017-3875-3 | |
received in 2017-05-03, accepted in 2017-12-04, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
BackgroundEphA2 is a crucial oncogene in gastric cancer (GC) development and metastasis, this study aims to identify microRNAs that target it and serve as key regulators of gastric carcinogenesis.MethodsWe identified several potential microRNAs targeting EphA2 by bioinformatics websites and then analyzed the role of miR-302b in modulating EphA2 in vitro and in vivo of GC, and it’s mechanism.ResultsOur analysis identified miR-302b, a novel regulator of EphA2, as one of the most significantly downregulated microRNA (miRNA) in GC tissues. Overexpression of miR-302b impaired GC cell migratory and invasive properties robustly and suppressed cell proliferation by arresting cells at G0–G1 phase in vitro. miR-302b exhibited anti-tumor activity by reversing EphA2 regulation, which relayed a signaling transduction cascade that attenuated the functions of N-cadherin, β-catenin, and Snail (markers of Wnt/β-catenin and epithelial-mesenchymal transition, EMT). This modulation of EphA2 also had distinct effects on cell proliferation and migration in GC in vivo.ConclusionsmiR-302b serves as a critical suppressor of GC cell tumorigenesis and metastasis by targeting the EphA2/Wnt/β-catenin/EMT pathway.
【 授权许可】
CC BY
© The Author(s). 2017
【 预 览 】
Files | Size | Format | View |
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RO202311090106165ZK.pdf | 7669KB | download |
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