BMC Infectious Diseases | |
Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting | |
Research Article | |
Andre G Loxton1  Gillian F Black1  Gerhard Walzl1  Novel N Chegou1  Kim Stanley1  Paulin N Essone1  Shreemanta K Parida2  Stefan HE Kaufmann2  T Mark Doherty3  Tom H Ottenhoff4  Michel R Klein4  Annemieke H Friggen4  Kees L Franken4  | |
[1] DST/NRF Centre of Excellence for Biomedical Tuberculosis Research and MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, P.O. Box 19063, 7505, Tygerberg, South Africa;Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, 10117, Berlin, Germany;Department of Infectious Disease Immunology, Statens Serum Institute, 2300s, Copenhagen, Denmark;GlaxoSmithKline, Nykær 68, Copenhagen, Denmark;Department of Infectious Diseases, Leiden University Medical Centre, P.O. Box 9600, 2300, Leiden, RC, The Netherlands; | |
关键词: Receiver Operating Characteristic; Tuberculin Skin Test; Peptide Pool; Receiver Operating Characteristic Analysis; General Discriminant Analysis; | |
DOI : 10.1186/1471-2334-12-10 | |
received in 2011-08-02, accepted in 2012-01-20, 发布年份 2012 | |
来源: Springer | |
【 摘 要 】
BackgroundConfirming tuberculosis (TB) disease in suspects in resource limited settings is challenging and calls for the development of more suitable diagnostic tools. Different Mycobacterium tuberculosis (M.tb) infection phase-dependent antigens may be differentially recognized in infected and diseased individuals and therefore useful as diagnostic tools for differentiating between M.tb infection states. In this study, we assessed the diagnostic potential of 118 different M.tb infection phase-dependent antigens in TB patients and household contacts (HHCs) in a high-burden setting.MethodsAntigens were evaluated using the 7-day whole blood culture technique in 23 pulmonary TB patients and in 19 to 21 HHCs (total n = 101), who were recruited from a high-TB incidence community in Cape Town, South Africa. Interferon-gamma (IFN-γ) levels in culture supernatants were determined by ELISA.ResultsEight classical TB vaccine candidate antigens, 51 DosR regulon encoded antigens, 23 TB reactivation antigens, 5 TB resuscitation promoting factors (rpfs), 6 starvation and 24 other stress response-associated TB antigens were evaluated in the study. The most promising antigens for ascertaining active TB were the rpfs (Rv0867c, Rv2389c, Rv2450c, Rv1009 and Rv1884c), with Areas under the receiver operating characteristics curves (AUCs) between 0.72 and 0.80. A combination of M.tb specific ESAT-6/CFP-10 fusion protein, Rv2624c and Rv0867c accurately predicted 73% of the TB patients and 80% of the non-TB cases after cross validation.ConclusionsIFN-γ responses to TB rpfs show promise as TB diagnostic candidates and should be evaluated further for discrimination between M.tb infection states.
【 授权许可】
CC BY
© Chegou et al; licensee BioMed Central Ltd. 2012
【 预 览 】
Files | Size | Format | View |
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RO202311090103579ZK.pdf | 312KB | download |
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