Gene family expansions and contractions are associated with host range in plant pathogens of the genus Colletotrichum

BMC Genomics

Research Article

Giovanni Vannacci¹  Antonio Zapparata¹  Sabrina Sarrocco¹  Daniel Buchvaldt Amby²  Surapareddy Sreenivasaprasad³  Michael R. Thon⁴  Serenella A. Sukno⁴  Gaétan Le Floch⁵  Riccardo Baroncelli⁵  Richard J. Harrison⁶  Eric Holub⁷ 

[1] Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy;Department of Plant and Environmental Sciences and Copenhagen Plant Science Centre, University of Copenhagen, Thorvaldsensvej 40, 1871 Frb. C, Copenhagen, Denmark;Institute of Biomedical and Environmental Science and Technology (iBEST), University of Bedfordshire, University Square, LU1 3JU, Luton, Bedfordshire, UK;Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), University of Salamanca, Campus de Villamayor, C/Del Duero, 12, 37185, Salamanca, Villamayor, Spain;Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), University of Western Brittany, Technopôle Brest-Iroise, 29280, Plouzané, France;NIAB-EMR, New Road, East Malling, ME19 6BJ, Kent, UK;School of Life Sciences, Warwick Crop Centre, University of Warwick, CV35 9EF, Wellesbourne, Warwickshire, UK;

关键词: CAZyme;    Plant pathogen;    Anthracnose;    Fungal genomics;    Colletotrichum;   

DOI  :  10.1186/s12864-016-2917-6

 received in 2016-04-14, accepted in 2016-07-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMany species belonging to the genus Colletotrichum cause anthracnose disease on a wide range of plant species. In addition to their economic impact, the genus Colletotrichum is a useful model for the study of the evolution of host specificity, speciation and reproductive behaviors. Genome projects of Colletotrichum species have already opened a new era for studying the evolution of pathogenesis in fungi.ResultsWe sequenced and annotated the genomes of four strains in the Colletotrichum acutatum species complex (CAsc), a clade of broad host range pathogens within the genus. The four CAsc proteomes and secretomes along with those representing an additional 13 species (six Colletotrichum spp. and seven other Sordariomycetes) were classified into protein families using a variety of tools. Hierarchical clustering of gene family and functional domain assignments, and phylogenetic analyses revealed lineage specific losses of carbohydrate-active enzymes (CAZymes) and proteases encoding genes in Colletotrichum species that have narrow host range as well as duplications of these families in the CAsc. We also found a lineage specific expansion of necrosis and ethylene-inducing peptide 1 (Nep1)-like protein (NLPs) families within the CAsc.ConclusionsThis study illustrates the plasticity of Colletotrichum genomes, and shows that major changes in host range are associated with relatively recent changes in gene content.

【 授权许可】

CC BY   
© The Author(s). 2016