Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts

BMC Plant Biology

Methodology Article

Jane A Langdale¹  Eftychios Frangedakis²  Péter Szövényi³  Mariana Ricca⁴  Dietmar Quandt⁵  Susann Wicke⁶ 

[1] Department of Plant Sciences, University of Oxford, South Parks Rd, Oxford, UK;Department of Plant Sciences, University of Oxford, South Parks Rd, Oxford, UK;Current Address: Graduate School of Science, University of Tokyo, 7-3-1 Hongo, 113 0033, Bunkyo-ku, Tokyo, Japan;Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland;Institute of Systematic Botany, University of Zurich, Zurich, Switzerland;Swiss Institute of Bioinformatics, Quartier Sorge-Batiment Genopode, Lausanne, Switzerland;MTA-ELTE-MTM Ecology Research Group, ELTE, Biological Institute, Budapest, Hungary;Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland;Swiss Institute of Bioinformatics, Quartier Sorge-Batiment Genopode, Lausanne, Switzerland;Nees-Institut für Biodiversität der Pflanzen, University of Bonn, Meckenheimer Allee 170, D – 53115, Bonn, Germany;Nees-Institut für Biodiversität der Pflanzen, University of Bonn, Meckenheimer Allee 170, D – 53115, Bonn, Germany;Institute for Evolution and Biodiversity, University of Muenster, Huefferstr. 1, 48149, Muenster, Germany;

关键词: Bryophytes;    Non-seed plants;    Model species;    Development;    Evolution;    Sporophyte;    Genetically divergent strains;   

DOI  :  10.1186/s12870-015-0481-x

 received in 2015-01-28, accepted in 2015-03-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPlants colonized terrestrial environments approximately 480 million years ago and have contributed significantly to the diversification of life on Earth. Phylogenetic analyses position a subset of charophyte algae as the sister group to land plants, and distinguish two land plant groups that diverged around 450 million years ago – the bryophytes and the vascular plants. Relationships between liverworts, mosses hornworts and vascular plants have proven difficult to resolve, and as such it is not clear which bryophyte lineage is the sister group to all other land plants and which is the sister to vascular plants. The lack of comparative molecular studies in representatives of all three lineages exacerbates this uncertainty. Such comparisons can be made between mosses and liverworts because representative model organisms are well established in these two bryophyte lineages. To date, however, a model hornwort species has not been available.ResultsHere we report the establishment of Anthoceros agrestis as a model hornwort species for laboratory experiments. Axenic culture conditions for maintenance and vegetative propagation have been determined, and treatments for the induction of sexual reproduction and sporophyte development have been established. In addition, protocols have been developed for the extraction of DNA and RNA that is of a quality suitable for molecular analyses. Analysis of haploid-derived genome sequence data of two A. agrestis isolates revealed single nucleotide polymorphisms at multiple loci, and thus these two strains are suitable starting material for classical genetic and mapping experiments.ConclusionsMethods and resources have been developed to enable A. agrestis to be used as a model species for developmental, molecular, genomic, and genetic studies. This advance provides an unprecedented opportunity to investigate the biology of hornworts.

【 授权许可】

Unknown   
© Szövényi et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.