Methylprednisolone blocks interleukin 1 beta induced calcitonin gene related peptide release in trigeminal ganglia cells

The Journal of Headache and Pain

Research Article

Ulrich Dirnagl¹  Lars Neeb¹  Uwe Reuter¹  Jan Hoffmann²  Peter Hellen³ 

[1]Department of Neurology and Experimental Neurology, Charité Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany

[2]Department of Neurology and Experimental Neurology, Charité Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany

[3]Department of Systems Neuroscience, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246, Hamburg, Germany

[4]Department of Neuroradiology, Universitätsmedizin Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany

关键词: Cluster headache;    Migraine;    Trigeminal ganglia cells;    Calcitonin gene related peptide;    Methylprednisolone;    Metoprolol;    Interleukin-1β;    Prostaglandin E;   

DOI  :  10.1186/s10194-016-0609-x

 received in 2015-12-28, accepted in 2016-02-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMethylprednisolone (MPD) is a rapid acting highly effective cluster headache preventive and also suppresses the recurrence of migraine attacks. Previously, we could demonstrate that elevated CGRP plasma levels in a cluster headache bout are normalized after a course of high dose corticosteroids. Here we assess whether MPD suppresses interleukin-1β (IL-1β)- and prostaglandin E2 (PGE2)-induced CGRP release in a cell culture model of trigeminal ganglia cells, which could account for the preventive effect in migraine and cluster headache. Metoprolol(MTP), a migraine preventive with a slow onset of action, was used for comparison.MethodsPrimary cultures of rat trigeminal ganglia were stimulated for 24 h with 10 ng/ml IL-1β or for 4 h with 10 μM PGE2 following the exposure to 10 or 100 μM MPD or 100 nM or 10 µM MTP for 45 min or 24 h. CGRP was determined by using a commercial enzyme immunoassay.ResultsMPD but not MTP blocked IL-1β-induced CGRP release from cultured trigeminal cells. PGE2-stimulated CGRP release from trigeminal ganglia cell culture was not affected by pre-stimulation whether with MPD or MTP.ConclusionMPD but not MTP suppresses cytokine (IL-1β)-induced CGRP release from trigeminal ganglia cells. We propose that blockade of cytokine mediated trigeminal activation may represent a potential mechanism of action that mediates the preventive effect of MTP on cluster headache and recurrent migraine attacks.

【 授权许可】

CC BY   
© Neeb et al. 2016