Reduced circulating endothelial progenitor cells in reversible cerebral vasoconstriction syndrome

The Journal of Headache and Pain

Research Article

Chin-Wen Chi¹  Po-Hsun Huang²  Yen-Feng Wang³  Shih-Pin Chen³  Jong-Ling Fuh⁴  Shuu-Jiun Wang⁴ 

[1] Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan;Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan;Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan;Department of Internal Medicine, Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan;Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan;Department of Neurology, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan;Brain Research Center, National Yang-Ming University, Taipei, Taiwan;Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan;Department of Neurology, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan;Brain Research Center, National Yang-Ming University, Taipei, Taiwan;Institute of Brain Science, National Yang-Ming University, Taipei, Taiwan;

关键词: Reversible cerebral vasoconstriction syndrome;    Thunderclap headaches;    Endothelial progenitor cells;    Endothelial dysfunction;    Cerebral arteries;   

DOI  :  10.1186/1129-2377-15-82

 received in 2014-09-28, accepted in 2014-11-20,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe pathophysiology of reversible cerebral vasoconstriction syndrome (RCVS) remains elusive. Endothelial dysfunction might play a role, but direct evidence is lacking. This study aimed to explore whether patients with RCVS have a reduced level of circulating circulating endothelial progenitor cells (EPCs) to repair the dysfunctional endothelial vasomotor control.MethodsWe prospectively recruited 24 patients with RCVS within one month of disease onset and 24 healthy age- and sex-matched controls. Flow cytometry was used to quantify the numbers of circulating EPCs, defined as KDR+CD133+, CD34+CD133+, and CD34+KDR+ double-positive mononuclear cells. The Lindegaard index, an index of vasoconstriction, was calculated by measuring the mean flow velocity of middle cerebral arteries and distal extracranial internal carotid arteries via color-coded sonography on the same day as blood drawing. A Lindegaard index of 2 was chosen as the cutoff value for significant vasoconstriction of middle cerebral arteries based on our previous study.ResultsPatients with RCVS had a reduced number of CD34+KDR+ cells (0.009 ± 0.006% vs. 0.014 ± 0.010%, p = 0.031) but not KDR+CD133+ cells or CD34+CD133+ EPCs, in comparison with controls. The number of CD34+KDR+ cells was inversely correlated with the Lindegaard index (rs = -0.418, p = 0.047). Of note, compared to controls, patients with a Lindegaard index > 2 (n = 13) had a reduced number of CD34+KDR+ cells (0.007 ± 0.005% vs. 0.014 ± 0.010%, p = 0.010), but those with a Lindegaard index ≤ 2 did not.ConclusionsPatients with RCVS had reduced circulating CD34+KDR+ EPCs, which were correlated with the severity of vasoconstriction. Endothelial dysfunction might contribute to the pathogenesis of RCVS.

【 授权许可】

Unknown   
© Chen et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.