The role of the transient receptor potential ankyrin type-1 (TRPA1) channel in migraine pain: evaluation in an animal model

The Journal of Headache and Pain

Research Article

Oscar Francesconi¹  Cristina Nativi²  Anna Maria Zanaboni³  Cristina Tassorelli³  Chiara Demartini³  Germana Tonsi³  Rosaria Greco⁴ 

[1] Department of Chemistry ‘Ugo Schiff’, University of Florence, Florence, Italy;Department of Chemistry ‘Ugo Schiff’, University of Florence, Florence, Italy;FiorGen, University of Florence, Florence, Italy;Laboratory of Neurophysiology of Integrative Autonomic Systems, Headache Science Center, “C. Mondino” National Neurological Institute, Pavia, Italy;Department of Brain and Behavioral Sciences University of Pavia, Pavia, Italy;Laboratory of Neurophysiology of Integrative Autonomic Systems, Headache Science Center, “C. Mondino” National Neurological Institute, Pavia, Italy;IRCCS “National Neurological Institute C. Mondino” Foundation, Via Mondino, 2, 27100, Pavia, Italy;

关键词: NTG;    Migraine;    Trigeminal hyperalgesia;    TRPA1 antagonist;   

DOI  :  10.1186/s10194-017-0804-4

 received in 2017-06-13, accepted in 2017-08-29,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundClinical and experimental studies have pointed to the possible involvement of the transient receptor potential ankyrin type-1 (TRPA1) channels in migraine pain. In this study, we aimed to further investigate the role of these channels in an animal model of migraine using a novel TRPA1 antagonist, ADM_12, as a probe.MethodsThe effects of ADM_12 on nitroglycerin-induced hyperalgesia at the trigeminal level were investigated in male rats using the quantification of nocifensive behavior in the orofacial formalin test. The expression levels of the genes coding for c-Fos, TRPA1, calcitonin gene-related peptide (CGRP) and substance P (SP) in peripheral and central areas relevant for migraine pain were analyzed. CGRP and SP protein immunoreactivity was also evaluated in trigeminal nucleus caudalis (TNC).ResultsIn rats bearing nitroglycerin-induced hyperalgesia, ADM_12 showed an anti-hyperalgesic effect in the second phase of the orofacial formalin test. This effect was associated to a significant inhibition of nitroglycerin-induced increase in c-Fos, TRPA1 and neuropeptides mRNA levels in medulla-pons area, in the cervical spinal cord and in the trigeminal ganglion. No differences between groups were seen as regards CGRP and SP protein expression in the TNC.ConclusionsThese findings support a critical involvement of TRPA1 channels in the pathophysiology of migraine, and show their active role in counteracting hyperalgesia at the trigeminal level.

【 授权许可】

CC BY   
© The Author(s). 2017