Genetic association and gene expression studies suggest that genetic variants in the SYNE1 and TNF genes are related to menstrual migraine

The Journal of Headache and Pain

Research Article

E Anne MacGregor¹  Alison Frith²  Rod A Lea³  Heidi Sutherland³  Astrid J Rodriguez-Acevedo³  Lyn R Griffiths³  Robert A Smith³  Bishakha Roy³ 

[1] Centre for Neuroscience & Trauma, Blizard Institute of Cell and Molecular Science, London, UK;Clinithink Limited, Bridgend, UK;Genomics Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, Musk Ave, Kelvin Grove, 4059, Brisbane, QLD, Australia;

关键词: Migraine;    Menstrual migraine (MM);    Menstrual related migraine (MRM);    Pure menstrual migraine (PMM);    ESR1;    PGR;    TNF;    SYNE1;   

DOI  :  10.1186/1129-2377-15-62

 received in 2014-08-18, accepted in 2014-08-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMenstrual migraine (MM) encompasses pure menstrual migraine (PMM) and menstrually-related migraine (MRM). This study was aimed at investigating genetic variants that are potentially related to MM, specifically undertaking genotyping and mRNA expression analysis of the ESR1, PGR, SYNE1 and TNF genes in MM cases and non-migraine controls.MethodsA total of 37 variants distributed across 14 genes were genotyped in 437 DNA samples (282 cases and 155 controls). In addition levels of gene expression were determined in 74 cDNA samples (41 cases and 33 controls). Association and correlation analysis were performed using Plink and RStudio.ResultsSNPs rs3093664 and rs9371601 in TNF and SYNE1 genes respectively, were significantly associated with migraine in the MM population (p = 0.008; p = 0.009 respectively). Analysis of qPCR results found no significant difference in levels of gene expression between cases and controls. However, we found a significant correlation between the expression of ESR1 and SYNE1, ESR1 and PGR and TNF and SYNE1 in samples taken during the follicular phase of the menstrual cycle.ConclusionsOur results show that SNPs rs9371601 and rs3093664 in the SYNE1 and TNF genes respectively, are associated with MM. The present study also provides strong evidence to support the correlation of ESR1, PGR, SYNE1 and TNF gene expression in MM.

【 授权许可】

Unknown   
© Rodriguez-Acevedo et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.