Insights into the role of three Endonuclease III enzymes for oxidative stress resistance in the extremely radiation resistant bacterium Deinococcus radiodurans

Frontiers in Microbiology

Microbiology

Filipe Rollo¹  Célia V. Romão¹  Guilherme D. Martins¹  André G. Gouveia¹  Elin Moe²  Solenne Ithurbide³  Pascale Servant³ 

[1] Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Oeiras, Portugal;Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Oeiras, Portugal;Department of Chemistry, UiT - The Arctic University of Norway, Tromsø, Norway;Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif sur Yvette, France;

关键词: DNA repair;    DNA glycosylase;    radiation damage;    base excision repair;    oxidation damage;   

DOI  :  10.3389/fmicb.2023.1266785

 received in 2023-07-25, accepted in 2023-08-29,  发布年份 2023

来源: Frontiers

【 摘 要 】

The extremely radiation and desiccation resistant bacterium Deinococcus radiodurans possesses three genes encoding Endonuclease III-like enzymes (DrEndoIII1, DrEndoIII2, DrEndoIII3). In vitro enzymatic activity measurements revealed that DrEndoIII2 is the main Endonuclease III in this organism, while DrEndoIII1 and 3 possess unusual and, so far, no detectable EndoIII activity, respectively. In order to understand the role of these enzymes at a cellular level, DrEndoIII knockout mutants were constructed and subjected to various oxidative stress related conditions. The results showed that the mutants are as resistant to ionizing and UV-C radiation as well as H2O2 exposure as the wild type. However, upon exposure to oxidative stress induced by methyl viologen, the knockout strains were more resistant than the wild type. The difference in resistance may be attributed to the observed upregulation of the EndoIII homologs gene expression upon addition of methyl viologen. In conclusion, our data suggest that all three EndoIII homologs are crucial for cell survival in stress conditions, since the knockout of one of the genes tend to be compensated for by overexpression of the genes encoding the other two.

【 授权许可】

Unknown   
Copyright © 2023 Rollo, Martins, Gouveia, Ithurbide, Servant, Romão and Moe.

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