Association between immunoglobulin G N-glycosylation and lupus nephritis in female patients with systemic lupus erythematosus: a case-control study

Frontiers in Immunology

Immunology

Qinqin Zhao¹  Yanjun Shi²  Yujing Zhang³  Liangao Wang³  Xinxia Lu³  Yingjie Wang³  Wei Wang⁴  Dong Li⁵  Long Ji⁶  Haifeng Hou⁷  Meng Wang⁸  Yuejin Li⁹ 

[1] Department of Geriatric Cognitive Medicine, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China;Department of Rheumatology and Immunology, Liaocheng People’s Hospital, Liao’cheng, China;School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;Centre for Precision Health, School of Medical and Health Sciences, Edith Cowan University, Perth, WA, Australia;School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;Clinical Research Center, The Second Affiliated Hospital of Shandong First Medical University, Tai’an, China;School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;College of Sports Medicine and Rehabilitation, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an, China;School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;Department of Gastroenterology, The Second Affiliated Hospital of Shandong First Medical University, Tai’an, China;School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;Jinshan District Center for Disease Control and Prevention, Shanghai, China;Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining, China;

关键词: immunoglobulin G;    N-glycosylation;    lupus nephritis;    inflammation;    biomarkers;   

DOI  :  10.3389/fimmu.2023.1257906

 received in 2023-07-13, accepted in 2023-09-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundLupus nephritis (LN) is a crucial complication of systemic lupus erythematosus (SLE) and has important clinical implications in guiding treatment. N-glycosylation of immunoglobulin G (IgG) plays a key role in the development of SLE by affecting the balance of anti-inflammatory and proinflammatory responses. This study aimed to evaluate the performance of IgG N-glycosylation for diagnosing LN in a sample of female SLE patients.MethodsThis case-control study recruited 188 women with SLE, including 94 patients with LN and 94 age-matched patients without LN. The profiles of plasma IgG N-glycans were detected by hydrophilic interaction chromatography with ultra-performance liquid chromatography (HILIC-UPLC). A multivariate logistic regression model was used to explore the associations between IgG N-glycans and LN. A diagnostic model was developed using the significant glycans as well as demographic factors. The performance of IgG N-glycans in the diagnosis of LN was evaluated by receiver operating characteristic (ROC) curve analysis, and the area under the curve (AUC) and its 95% confidence interval (CI) were calculated.ResultsThere were significant differences in 9 initial glycans (GP2, GP4, GP6, GP8, GP10, GP14, GP16, GP18 and GP23) between women with SLE with and without LN (P < 0.05). The levels of sialylated, galactosylated and fucosylated glycans were significantly lower in the LN patients than in the control group, while bisected N-acetylglucosamine (GlcNAc) glycans were increased in LN patients (P < 0.05). GP8, GP10, GP18, and anemia were included in our diagnostic model, which performed well in differentiating female SLE patients with LN from those without LN (AUC = 0.792, 95% CI: 0.727 to 0.858).ConclusionOur findings indicate that decreased sialylation, galactosylation, and core fucosylation and increased bisecting GlcNAc might play a role in the development of LN by upregulating the proinflammatory response of IgG. IgG N-glycans can serve as potential biomarkers to differentiate individuals with LN among SLE patients.

【 授权许可】

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Copyright © 2023 Lu, Wang, Wang, Li, Zhao, Shi, Zhang, Wang, Wang, Ji, Hou and Li