Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Daisaku Sasaki¹  Akari Takagi²  Yuya Uejima²  Misato Nagao²  Munehiko Asayama³ 

[1] BioX Chemical Industries Co., Ltd., Hiroshima, Japan;College of Agriculture, Ibaraki University, Ibaraki, Japan;College of Agriculture, Ibaraki University, Ibaraki, Japan;United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan;

关键词: algae;    biorefinery;    carbohydrate;    lipid;    Parachlorella;    polysaccharide;   

DOI  :  10.3389/fbioe.2023.1233944

 received in 2023-06-03, accepted in 2023-08-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Efficient pH and dissolved CO2 conditions for indoor (50–450 mL scale) and outdoor (100–500 L scale) culture of a green alga BX1.5 strain that can produce useful intracellular lipids and extracellular polysaccharides were investigated for the first time in Parachlorella sp. The cultures harvested under 26 different conditions were analysed for pH, dissolved CO2 concentration, and the biomass of extracellular polysaccharides. The BX1.5 strain could thrive in a wide range of initial medium pH ranging from 3 to 11 and produced valuable lipids such as C16:0, C18:2, and C18:3 under indoor and outdoor culture conditions when supplied with 2.0% dissolved CO2. Particularly, the acidic BG11 medium effectively increased the biomass of extracellular polysaccharides during short-term outdoor cultivation. The BG11 liquid medium also led to extracellular polysaccharide production, independent of acidity and alkalinity, proportional to the increase in total sugars derived from cells supplied with high CO2 concentrations. These results suggest Parachlorella as a promising strain for indoor and outdoor cultivation to produce valuable materials.

【 授权许可】

Unknown   
Copyright © 2023 Takagi, Nagao, Uejima, Sasaki and Asayama.