Identification of southern corn rust resistance QTNs in Chinese summer maize germplasm via multi-locus GWAS and post-GWAS analysis

Frontiers in Plant Science

Plant Science

Ting Li¹  Guoping Shu¹  Aifang Wang¹  Junqiang Ding²  Ruijie Chen³  Fei Gao³  Xingchuan Wang³  Yibo Wang³  Aifen Wang³ 

[1] Center of Biotechnology, Beijing Lantron Seed, LongPing High-tech Corp., Zhengzhou, Henan, China;College of Agronomy, Henan Agricultural University, Zhengzhou, Henan, China;Henan LongPing-Lantron AgriScience & Technology Co., LTD, Zhengzhou, LongPing High-tech Corp., Zhengzhou, Henan, China;

关键词: maize;    multi-locus GWAS;    QTNs;    post-gwas;    case-control;    disease resistance gene;    southern corn rust (SCR);    Puccinia polysora;   

DOI  :  10.3389/fpls.2023.1221395

 received in 2023-05-12, accepted in 2023-08-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

Southern corn rust (SCR) caused by Puccinia polysora Underw is a major disease leading to severe yield losses in China Summer Corn Belt. Using six multi-locus GWAS methods, we identified a set of SCR resistance QTNs from a diversity panel of 140 inbred lines collected from China Summer Corn Belt. Thirteen QTNs on chromosomes 1, 2, 4, 5, 6, and 8 were grouped into three types of allele effects and their associations with SCR phenotypes were verified by post-GWAS case-control sampling, allele/haplotype effect analysis. Relative resistance (RRR) and relative susceptibility (RRs) catering to its inbred carrier were estimated from single QTN and QTN-QTN combos and epistatitic effects were estimated for QTN-QTN combos. By transcriptomic annotation, a set of candidate genes were predicted to be involved in transcriptional regulation (S5_145, Zm00001d01613, transcription factor GTE4), phosphorylation (S8_123, Zm00001d010672, Pgk2- phosphoglycerate kinase 2), and temperature stress response (S6_164a/S6_164b, Zm00001d038806, hsp101, and S5_211, Zm00001d017978, cellulase25). The breeding implications of the above findings were discussed.

【 授权许可】

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Copyright © 2023 Shu, Wang, Wang, Ding, Chen, Gao, Wang, Li and Wang