Effects of stress conditions on plasma parameters and gene expression in the skin mucus of farmed rainbow trout (Oncorhynchus mykiss)

Frontiers in Veterinary Science

Veterinary Science

Adina Friedl¹  Mansour El-Matbouli¹  Mona Saleh¹  Alessandra Roncarati²  Gian Enrico Magi²  Elisa Fiordelmondo² 

[1] Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria;School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy;

关键词: fish welfare;    fish farming;    qRT-PCR;    cortisol;    IL-6;   

DOI  :  10.3389/fvets.2023.1183246

 received in 2023-03-09, accepted in 2023-08-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

The aim of this study was to investigate the physiological response of rainbow trout (Oncorhynchus mykiss) before slaughtering in the last phase of farming analyzing skin mucus and plasma. Two groups of rainbow trout were considered: Group UN (“unstressed”), represented by fish randomly captured from raceways, in the last phase of a standard fattening cycle; Group S (“stressed”), collected at the end of the pre-slaughtering tank, soon after slaughtering. The fish skin mucus was swabbed from head to tail using a sterile plastic spatula and the blood was collected through an endocardial puncture. qRT-PCR was used to study the gene expression in skin mucus. The mRNA expression levels of the IL-6 and IgD genes were higher in the S than in the Group UN. The plasma analysis showed an only a decrease in the glucose plasma levels in the Group S when compared to the Group UN. The present results indicated that the procedures adopted after slaughtering only affected changes in plasma glucose and skin mucus activity in rainbow trout suggesting that management protocol was compatible with non-stressful farming conditions.

【 授权许可】

Unknown   
Copyright © 2023 Fiordelmondo, Magi, Friedl, El-Matbouli, Roncarati and Saleh.