Kre6 (yeast 1,6-β-transglycosylase) homolog, PhTGS, is essential for β-glucan synthesis in the haptophyte Pleurochrysis haptonemofera

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Hirotoshi Endo¹  Koki Asakawa²  Mayuka Inukai²  Shoko Fujiwara²  Keisuke Amano²  Yuki Yasuda²  Naoya Kobayashi²  Steven Ball³  Christophe Colleoni³  Ugo Cenci³ 

[1] National Institute of Technology, Tsuruoka College, Tsuruoka, Japan;School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan;University of Lille, French National Centre for Scientific Research, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France;

关键词: β-glucan;    coccolithophorid;    glucan synthase;    Haptophyta;    storage glucan;    transglycosylase;   

DOI  :  10.3389/fbioe.2023.1259587

 received in 2023-07-18, accepted in 2023-08-29,  发布年份 2023

来源: Frontiers

【 摘 要 】

Haptophytes synthesize unique β-glucans containing more β-1,6-linkages than β-1,3 linkages, as a storage polysaccharide. To understand the mechanism of the synthesis, we investigated the roles of Kre6 (yeast 1,6-β-transglycosylase) homologs, PhTGS, in the haptophyte Pleurochrysis haptonemofera. RNAi of PhTGS repressed β-glucan accumulation and simultaneously induced lipid production, suggesting that PhTGS is involved in β-glucan synthesis and that the knockdown leads to the alteration of the carbon metabolic flow. PhTGS was expressed more in light, where β-glucan was actively produced by photosynthesis, than in the dark. The crude extract of E. coli expressing PhKre6 demonstrated its activity to incorporate 14C-UDP-glucose into β-glucan of P. haptonemofera. These findings suggest that PhTGS functions in storage β-glucan synthesis specifically in light, probably by producing the β-1,6-branch.

【 授权许可】

Unknown   
Copyright © 2023 Inukai, Kobayashi, Endo, Asakawa, Amano, Yasuda, Cenci, Colleoni, Ball and Fujiwara.