Frontiers in Bioengineering and Biotechnology | |
Kre6 (yeast 1,6-β-transglycosylase) homolog, PhTGS, is essential for β-glucan synthesis in the haptophyte Pleurochrysis haptonemofera | |
Bioengineering and Biotechnology | |
Hirotoshi Endo1  Koki Asakawa2  Mayuka Inukai2  Shoko Fujiwara2  Keisuke Amano2  Yuki Yasuda2  Naoya Kobayashi2  Steven Ball3  Christophe Colleoni3  Ugo Cenci3  | |
[1] National Institute of Technology, Tsuruoka College, Tsuruoka, Japan;School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan;University of Lille, French National Centre for Scientific Research, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France; | |
关键词: β-glucan; coccolithophorid; glucan synthase; Haptophyta; storage glucan; transglycosylase; | |
DOI : 10.3389/fbioe.2023.1259587 | |
received in 2023-07-18, accepted in 2023-08-29, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Haptophytes synthesize unique β-glucans containing more β-1,6-linkages than β-1,3 linkages, as a storage polysaccharide. To understand the mechanism of the synthesis, we investigated the roles of Kre6 (yeast 1,6-β-transglycosylase) homologs, PhTGS, in the haptophyte Pleurochrysis haptonemofera. RNAi of PhTGS repressed β-glucan accumulation and simultaneously induced lipid production, suggesting that PhTGS is involved in β-glucan synthesis and that the knockdown leads to the alteration of the carbon metabolic flow. PhTGS was expressed more in light, where β-glucan was actively produced by photosynthesis, than in the dark. The crude extract of E. coli expressing PhKre6 demonstrated its activity to incorporate 14C-UDP-glucose into β-glucan of P. haptonemofera. These findings suggest that PhTGS functions in storage β-glucan synthesis specifically in light, probably by producing the β-1,6-branch.
【 授权许可】
Unknown
Copyright © 2023 Inukai, Kobayashi, Endo, Asakawa, Amano, Yasuda, Cenci, Colleoni, Ball and Fujiwara.
【 预 览 】
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RO202310128386644ZK.pdf | 1602KB | download |