Overcoming extended lag phase on optically pure lactic acid production from pretreated softwood solids

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Henrik Almqvist¹  Joana Campos²  Ola Wallberg²  Gunnar Lidén²  Jie Bao³ 

[1] CR Competence AB, Lund, Sweden;Department of Chemical Engineering, Lund University, Lund, Sweden;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China;

关键词: D-lactic acid;    Pediococcus acidilactici;    softwood;    cell-solid interaction;    lag phase;    inhibition;   

DOI  :  10.3389/fbioe.2023.1248441

 received in 2023-06-27, accepted in 2023-08-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Optically pure lactic acid (LA) is needed in PLA (poly-lactic acid) production to build a crystalline structure with a higher melting point of the biopolymer than that of the racemic mixture. Lignocellulosic biomass can be used as raw material for LA production, in a non-food biorefinery concept. In the present study, genetically engineered P. acidilactici ZP26 was cultivated in a simultaneous saccharification and fermentation (SSF) process using steam pretreated softwood solids as a carbon source to produce optically pure D-LA. Given the low concentrations of identifiable inhibitory compounds from sugar and lignin degradation, the fermentation rate was expected to follow the rate of enzymatic hydrolysis. However, added pretreated solids (7% on weight (w/w) of water-insoluble solids [WIS]) significantly and immediately affected the process performance, which resulted in a long lag phase (more than 40 h) before the onset of the exponential phase of the fermentation. This unexpected delay was also observed without the addition of enzymes in the SSF and in a model fermentation with glucose and pretreated solids without added enzymes. Experiments showed that it was possible to overcome the extended lag phase in the presence of pretreated softwood solids by allowing the microorganism to initiate its exponential phase in synthetic medium, and subsequently adding the softwood solids and enzymatic blend to proceed to an SSF with D-LA production.

【 授权许可】

Unknown   
Copyright © 2023 Campos, Almqvist, Bao, Wallberg and Lidén.