Characterisation and development of histopathological lesions in a guinea pig model of Mycobacterium tuberculosis infection

Frontiers in Veterinary Science

Veterinary Science

Irene Agulló-Ros¹  Fernanda Larenas-Muñoz¹  Inés Ruedas-Torres²  Rebecca Winsbury³  Alison Bird³  Francisco J. Salguero³  Simon Clark³  Emma Rayner³  Laura Hunter⁴ 

[1] Department of Anatomy and Comparative Pathology and Toxicology, Pathology and Immunology Group (UCO-PIG), UIC Zoonosis y Enfermedades Emergentes ENZOEM, University of Córdoba, International Excellence Agrifood Campus ‘CeiA3’, Córdoba, Spain;Department of Anatomy and Comparative Pathology and Toxicology, Pathology and Immunology Group (UCO-PIG), UIC Zoonosis y Enfermedades Emergentes ENZOEM, University of Córdoba, International Excellence Agrifood Campus ‘CeiA3’, Córdoba, Spain;Pathology Department, UK Health Security Agency (UKHSA), Porton Down, Salisbury, United Kingdom;Pathology Department, UK Health Security Agency (UKHSA), Porton Down, Salisbury, United Kingdom;Pathology Department, UK Health Security Agency (UKHSA), Porton Down, Salisbury, United Kingdom;School of Biosciences and Medicine, University of Surrey, Guildford, United Kingdom;

关键词: tuberculosis;    guinea pig;    granuloma;    pathology, animal model;    Mycobacterium tuberculosis;   

DOI  :  10.3389/fvets.2023.1264200

 received in 2023-07-20, accepted in 2023-09-08,  发布年份 2023

来源: Frontiers

【 摘 要 】

Tuberculosis (TB) remains a very significant infectious disease worldwide. New vaccines and therapies are needed, even more crucially with the increase of multi-drug resistant Mycobacterium tuberculosis strains. Preclinical animal models are very valuable for the development of these new disease control strategies. Guinea pigs are one of the best models of TB, sharing many features with the pathology observed in human TB. Here we describe the development of TB lesions in a guinea pig model of infection. We characterise the granulomatous lesions in four developmental stages (I–IV), using histopathological analysis and immunohistochemical (IHC) techniques to study macrophages, T cells, B cells and granulocytes. The granulomas in the guinea pigs start as aggregations of macrophages and few heterophils, evolving to larger lesions showing central caseous necrosis with mineralisation and abundant acid-fast bacilli, surrounded by a rim of macrophages and lymphocytes in the outer layers of the granuloma. Multinucleated giant cells are very rare and fibrotic capsules are not formed in this animal model.

【 授权许可】

Unknown   
Copyright © 2023 Larenas-Muñoz, Ruedas-Torres, Hunter, Bird, Agulló-Ros, Winsbury, Clark, Rayner and Salguero.