Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group

Frontiers in Plant Science

Plant Science

Rodomiro Ortiz¹  Raúl Blas²  Germán De la Cruz³  Willmer Pérez⁴  Edgar Neyra⁵ 

[1] Department of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, Sweden;Instituto de Biotecnologia (IBT), Facultad de Agronomia, Universidad Nacional Agraria La Molina (UNALM), Lima, Peru;Laboratorio de Genética y Biotecnología Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de San Cristóbal de Huamanga (UNSCH), Ayacucho, Peru;Plant Pathology Laboratory, Crop and Systems Sciences Division, International Potato Center, Lima, Peru;Unidad de Genómica, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia, Lima, Peru;Departamento Académico de Tecnología Médica, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Peru;

关键词: gene expression;    genome;    host plant resistance;    Phytophthora infestans;    Solanum tuberosum;   

DOI  :  10.3389/fpls.2023.1210046

 received in 2023-04-21, accepted in 2023-08-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Characterization of major resistance (R) genes to late blight (LB) –caused by the oomycete Phytophthora infestans– is very important for potato breeding. The objective of this study was to identify novel genes for resistance to LB from diploid Solanum tuberosum L. Andigenum Group (StAG) cultivar accessions. Using comparative analysis with a edgeR bioconductor package for differential expression analysis of transcriptomes, two of these accessions with contrasting levels of resistance to LB were analyzed using digital gene expression data. As a result, various differentially expressed genes (P ≤ 0.0001, Log2FC ≥ 2, FDR < 0.001) were noted. The combination of transcriptomic analysis provided 303 candidate genes that are overexpressed and underexpressed, thereby giving high resistance to LB. The functional analysis showed differential expression of R genes and their corresponding proteins related to disease resistance, NBS-LRR domain proteins, and specific disease resistance proteins. Comparative analysis of specific tissue transcriptomes in resistant and susceptible genotypes can be used for rapidly identifying candidate R genes, thus adding novel genes from diploid StAG cultivar accessions for host plant resistance to P. infestans in potato.

【 授权许可】

Unknown   
Copyright © 2023 De la Cruz, Blas, Pérez, Neyra and Ortiz