Identification of a novel locus C2 controlling canary yellow flesh color in watermelons

Frontiers in Genetics

Genetics

Gaeun Jang¹  Jagyeong Shin¹  Durre Shahwar¹  Girim Park¹  Younghoon Park²  Hoytaek Kim³  Chang Oh Hong⁴  Oakjin Lee⁵  Younjae Kim⁶  Gibeom Kwon⁶  Bingkui Jin⁷ 

[1] Department of Horticultural Bioscience, Pusan National University, Miryang, Republic of Korea;Department of Horticultural Bioscience, Pusan National University, Miryang, Republic of Korea;Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea;Department of Horticulture, Sunchon National University, Sunchon, Republic of Korea;Department of Life Science and Environmental Biochemistry, Pusan National University, Miryang, Republic of Korea;Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea;National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, Republic of Korea;Partner Seeds Co., Ltd., Gimje, Republic of Korea;UNELL Biotechnology Co., Ltd., Weifang, China;

关键词: Citrullus lanatus;    flesh color;    pentatricopeptide repeat;    marker-assisted selection;    watermelon;   

DOI  :  10.3389/fgene.2023.1256627

 received in 2023-07-11, accepted in 2023-08-29,  发布年份 2023

来源: Frontiers

【 摘 要 】

The flesh color of watermelon is an important trait that is determined by carotenoid composition and affects consumers’ fruit desirability. Although a complete dominant control by C locus (Cllcyb) for canary yellow flesh (CY) over red flesh has been reported, red and CY colors frequently appear as a mixed pattern in the same flesh (incomplete canary yellow, ICY) in F1 and inbred lines carrying dominant C alleles. Therefore, we examined the genetic control of the mixed color pattern in ICY using whole-genome resequencing of three ICY (ICY group) and three CY inbred lines (CY group), as well as genetic linkage mapping of an F2 population. The segregation pattern in 135 F2 plants indicated that CY is controlled by a single locus (named C2) dominant over ICY. The whole-genome resequencing of ICY and CY inbred lines revealed an ICY/CY-specific region of approximately 27.60–27.88 Mb on Chr. 2 that was polymorphic between the ICY and CY groups. Our genetic map, using nine cleaved amplified polymorphic sequence markers developed based on the single-nucleotide polymorphisms from the ICY/CY-specific region, confirmed that C2 is located on Chr. 2 and cosegregated with the marker (M7) derived from a non-synonymous single-nucleotide polymorphism of the pentatricopeptide repeat (PPR) gene (ClPPR, Cla97C02G039880). Additionally, 27 watermelon inbred lines of ICY, CY, and red flesh were evaluated using previously reported Cllcyb (C locus)-based markers and our C2 locus-linked ClPPR-based marker (M7). As a result, dominant alleles at the C2 locus were required to produce CY, in addition to dominant alleles at the C locus, while a recessive homozygous genotype at the C locus gave the red flesh irrespective of the genotype at the C2 locus. Using a ClPPR-based cleaved amplified polymorphic sequence developed in this study and Cllcyb-based markers, watermelon cultivars with CY, ICY, and red flesh could be successfully discerned, implying that the combined use of these markers will be efficient for marker-assisted selection of flesh color in watermelon breeding.

【 授权许可】

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Copyright © 2023 Park, Shahwar, Jang, Shin, Kwon, Kim, Hong, Jin, Kim, Lee and Park.