Identification and verification of a prognostic signature based on a miRNA–mRNA interaction pattern in colon adenocarcinoma

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Xiaonan Shen¹  Wenqing Jia²  Shuiyu Xu²  Haosheng Li²  Zichao Guo²  Changgang Wang²  Qiwu Zhao²  Wenchang Li³  Sixia Chen⁴ 

[1]Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

[2]Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

[3]Department of Interventional Radiography, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

[4]Tongji Hospital, Tongji University School of Medicine, Tongji University, Shanghai, China

关键词: colon adenocarcinoma;    miRNAs;    prognostic signature;    RNA therapy;    tissue microarray;   

DOI  :  10.3389/fcell.2023.1161667

 received in 2023-02-08, accepted in 2023-08-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

The expression characteristics of non-coding RNA (ncRNA) in colon adenocarcinoma (COAD) are involved in regulating various biological processes. To achieve these functions, ncRNA and a member of the Argonaute protein family form an RNA-induced silencing complex (RISC). The RISC is directed by ncRNA, especially microRNA (miRNA), to bind the target complementary mRNAs and regulate their expression by interfering with mRNA cleavage, degradation, or translation. However, how to identify potential miRNA biomarkers and therapeutic targets remains unclear. Here, we performed differential gene screening based on The Cancer Genome Atlas dataset and annotated meaningful differential genes to enrich related biological processes and regulatory cancer pathways. According to the overlap between the screened differential mRNAs and differential miRNAs, a prognosis model based on a least absolute shrinkage and selection operator-based Cox proportional hazards regression analysis can be established to obtain better prognosis characteristics. To further explore the therapeutic potential of miRNA as a target of mRNA intervention, we conducted an immunohistochemical analysis and evaluated the expression level in the tissue microarray of 100 colorectal cancer patients. The results demonstrated that the expression level of POU4F1, DNASE1L2, and WDR72 in the signature was significantly upregulated in COAD and correlated with poor prognosis. Establishing a prognostic signature based on miRNA target genes will help elucidate the molecular pathogenesis of COAD and provide novel potential targets for RNA therapy.

【 授权许可】

Unknown   
Copyright © 2023 Zhao, Li, Li, Guo, Jia, Xu, Chen, Shen and Wang.