Macropinocytosis in Gracilariopsis lemaneiformis (Rhodophyta)

Frontiers in Plant Science

Plant Science

Yiyi Hu¹  Baoheng Xiao¹  Pingping Li¹  Qiong Wu¹  Xiaoqing Feng¹  Jingyu Zhang¹  Jingru Yin¹  Zhenghong Sui¹  Guanpin Yang²  Haihong Chen³ 

[1] Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China;Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China;Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China;Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China;Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China;

关键词: macropinocytosis;    endocytosis;    Gracilariopsis lemaneiformis;    red alga;    F-actin;    PI3Ks;    small GTPase;   

DOI  :  10.3389/fpls.2023.1225675

 received in 2023-05-19, accepted in 2023-09-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Macropinocytosis is an endocytic process that plays an important role in animal development and disease occurrence but until now has been rarely reported in organisms with cell walls. We investigated the properties of endocytosis in a red alga, Gracilariopsis lemaneiformis. The cells non-selectively internalized extracellular fluid into large-scale endocytic vesicles (1.94 ± 0.51 μm), and this process could be inhibited by 5-(N-ethyl-N-isopropyl) amiloride, an macropinocytosis inhibitor. Moreover, endocytosis was driven by F-actin, which promotes formation of ruffles and cups from the cell surface and facilitates formation of endocytotic vesicles. After vesicle formation, endocytic vesicles could be acidified and acquire digestive function. These results indicated macropinocytosis in G. lemaneiformis. Abundant phosphatidylinositol kinase and small GTPase encoding genes were found in the genome of this alga, while PI3K, Ras, and Rab5, the important participators of traditional macropinocytosis, seem to be lacked. Such findings provide a new insight into endocytosis in organisms with cell walls and facilitate further research into the core regulatory mechanisms and evolution of macropinocytosis.

【 授权许可】

Unknown   
Copyright © 2023 Chen, Hu, Yang, Li, Yin, Feng, Wu, Zhang, Xiao and Sui