Lipid modification to improve cryotolerance of gametes, embryos and larvae and its potential application in aquaculture species: a review

Frontiers in Marine Science

Marine Science

Yingying Zhao¹  Youhong Tang²  Jianguang Qin²  Xiaoxu Li³  Xiaochen Zhu³  Yibing Liu⁴  Penny Miller-Ezzy⁵ 

[1] College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China;College of Science and Engineering, Flinders University, Adelaide, SA, Australia;College of Science and Engineering, Flinders University, Adelaide, SA, Australia;South Australian Research and Development Institute, Aquatic Science Centre, Adelaide, SA, Australia;Fisheries College, Ocean University of China, Qingdao, China;South Australian Research and Development Institute, Aquatic Science Centre, Adelaide, SA, Australia;

关键词: cryopreservation;    lipid application;    sperm;    oocyte;    embryo;    larva;    aquaculture;   

DOI  :  10.3389/fmars.2023.1235958

 received in 2023-06-07, accepted in 2023-08-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Cryopreservation is a technique to maintain biological materials’ physiological and genetic stability at an ultralow temperature. For commercially important livestock or aquatic species, gamete and embryo cryopreservation could play a significant role in breeding programs and commercial production. For example, it could help overcome key problems such as asynchronous maturation and an unbalanced sex ratio. However, the physiochemical stresses imposed by cryopreservation can negatively affect gametes and embryos, leading to a poor survival rate. Recent studies on cryoinjury have demonstrated that the cryosensitivity of lipids is one of the key causes of cryodamage in mammalians, as lipid compositions in membranes of gametes and embryos are closely related to their cryoresistance. In addition, the cryotolerance of gametes and embryos in some mammalian species has been improved by lipid modification. However, studies on the role of lipids in the cryopreservation of gametes, embryos, and larvae are rare in fish and shellfish. Therefore, this review focuses on recent methodological advances to improve cryotolerance by lipid modification, including lipid application or manipulation in human and livestock sperm, oocytes, and embryos, and how these novel approaches could improve cryopreservation techniques in aquatic species, especially for oocytes and embryos.

【 授权许可】

Unknown   
Copyright © 2023 Zhu, Miller-Ezzy, Zhao, Qin, Tang, Liu and Li