Hybrid Boolean gates show that Cas12c controls transcription activation effectively in the yeast S. cerevisiae

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Huanhuan Ge¹  Yifan Liu¹  Mario Andrea Marchisio² 

[1] School of Pharmaceutical Science and Engineering, Tianjin University, Tianjin, China;[e-mail];

关键词: CRISPR-Cas12c;    hybrid Boolean gates;    riboswitches;    saccharomyces cerevisiae;    anti-CRISPR;   

DOI  :  10.3389/fbioe.2023.1267174

 received in 2023-07-26, accepted in 2023-08-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

Among CRISPR-Cas systems, type V CRISPR-Cas12c is of significant interest because Cas12c recognizes a very simple PAM (TN) and has the ability to silence gene expression without cleaving the DNA. We studied how new transcription factors for the yeast Saccharomyces cerevisiae can be built on Cas12c. We found that, upon fusion to a strong activation domain, Cas12c is an efficient activator. Its functionality was proved as a component of hybrid Boolean gates, i.e., logic circuits that mix transcriptional and translational control (the latter reached via tetracycline-responsive riboswitches). Moreover, Cas12c activity can be strongly inhibited by the anti-CRISPR AcrVA1 protein. Thus, Cas12c has the potential to be a new tool to control the activation of gene expression within yeast synthetic gene circuits.

【 授权许可】

Unknown   
Copyright © 2023 Liu, Ge and Marchisio.