Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker

Frontiers in Oncology

Oncology

Jofy Devasia Puthenpurackal¹  Riaz Abdulla¹  Punchappady Devasya Rekha²  Sneha M. Pinto³  Yashwanth Subbannayya³ 

[1] Department of Oral Pathology and Microbiology, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangalore, India;Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, India;Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, India;School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom;

关键词: biomarkers;    early detection;    liquid biopsy;    autoantibodies;    screening biomarkers;   

DOI  :  10.3389/fonc.2023.1167691

 received in 2023-02-16, accepted in 2023-08-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionOral Squamous Cell Carcinoma (OSCC), a common malignancy of the head and neck region, is frequently diagnosed at advanced stages, necessitating the development of efficient diagnostic methods. Profiling autoantibodies generated against tumor-associated antigens have lately demonstrated a promising role in diagnosis, predicting disease course, and response to therapeutics and relapse.MethodsIn the current study, we, for the first time, aimed to identify and evaluate the diagnostic value of autoantibodies in serum samples of patients with OSCC using autoantibody profiling by an immunome protein array. The utility of anti-NUBP2 antibody and tissue positivity in OSCC was further evaluated.Results and discussionWe identified a total of 53 autoantibodies with significant differential levels between OSCC and control groups, including 25 that were increased in OSCC and 28 that were decreased. These included autoantibodies against Thymidine kinase 1 (TK1), nucleotide-binding protein 2 (NUBP2), and protein pyrroline-5-carboxylate reductase 1 (PYCR1), among others. Immunohistochemical validation indicated positive staining of NUBP2 in a large majority of cases (72%). Further, analysis of OSCC data available in TCGA revealed higher NUBP2 expression correlated with better disease-free patient survival. In conclusion, the differential serum autoantibodies identified in the current study, including those for NUBP2, could be used as potential biomarkers for early diagnosis or as screening biomarkers for OSCC pending investigation in a larger cohort.

【 授权许可】

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Copyright © 2023 Abdulla, Devasia Puthenpurackal, Pinto, Rekha and Subbannayya