T cell memory revisited using single telomere length analysis

Frontiers in Immunology

Immunology

Julia W. Grimstead¹  Duncan M. Baird¹  Kelly L. Miners²  Laureline Roger²  Kristin Ladell²  Louise Leonard²  David A. Price³ 

[1] Division of Cancer and Genetics, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom;Division of Infection and Immunity, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom;Division of Infection and Immunity, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom;Systems Immunity Research Institute, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom;

关键词: replicative history;    T cell differentiation;    T cell memory;    T cell senescence;    telomere length (TL);   

DOI  :  10.3389/fimmu.2023.1100535

 received in 2022-11-16, accepted in 2023-06-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

The fundamental basis of T cell memory remains elusive. It is established that antigen stimulation drives clonal proliferation and differentiation, but the relationship between cellular phenotype, replicative history, and longevity, which is likely essential for durable memory, has proven difficult to elucidate. To address these issues, we used conventional markers of differentiation to identify and isolate various subsets of CD8+ memory T cells and measured telomere lengths in these phenotypically defined populations using the most sensitive technique developed to date, namely single telomere length analysis (STELA). Naive cells were excluded on the basis of dual expression of CCR7 and CD45RA. Memory subsets were sorted as CD27+CD45RA+, CD27intCD45RA+, CD27−CD45RA+, CD27+CD45RAint, CD27−CD45RAint, CD27+CD45RA−, and CD27−CD45RA− at >98% purity. The shortest median telomere lengths were detected among subsets that lacked expression of CD45RA, and the longest median telomere lengths were detected among subsets that expressed CD45RA. Longer median telomere lengths were also a feature of subsets that expressed CD27 in compartments defined by the absence or presence of CD45RA. Collectively, these data suggested a disconnect between replicative history and CD8+ memory T cell differentiation, which is classically thought to be a linear process that culminates with revertant expression of CD45RA.

【 授权许可】

Unknown   
Copyright © 2023 Roger, Miners, Leonard, Grimstead, Price, Baird and Ladell