Deciphering the underlying immune network of the potato defense response inhibition by Phytophthora infestans nuclear effector Pi07586 through transcriptome analysis

Frontiers in Plant Science

Plant Science

Die Zhang¹  Shengnan Chen¹  Di Zhao¹  Hongyang Wang¹  Lan Yuan¹  Yumeng Xiong² 

[1] Yunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, China;Yunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, China;School of Life Science, Yunnan Normal University, Kunming, China;

关键词: potato;    late blight;    Phytophthora infestans;    effector;    RNA-seq;   

DOI  :  10.3389/fpls.2023.1269959

 received in 2023-07-31, accepted in 2023-09-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Phytophthora infestans, a highly destructive plant oomycete pathogen, is responsible for causing late blight in potatoes worldwide. To successfully infect host cells and evade immunity, P. infestans secretes various effectors into host cells and exclusively targets the host nucleus. However, the precise mechanisms by which these effectors manipulate host gene expression and reprogram defenses remain poorly understood. In this study, we focused on a nuclear-targeted effector, Pi07586, which has been implicated in immune suppression. Quantitative real-time PCR (qRT-PCR) analysis showed Pi07586 was significant up-regulation during the early stages of infection. Agrobacterium-induced transient expression revealed that Pi07586 localized in the nucleus of leaf cells. Overexpression of Pi07586 resulted in increased leaf colonization by P. infestans. RNA-seq analysis revealed that Pi07586 effectively suppressed the expression of PR-1C-like and photosynthetic antenna protein genes. Furthermore, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS) analysis indicated that Pi07586 overexpression led to a substantial decrease in abscisic acid (ABA), jasmonic acid (JA), and jasmonoyl-isoleucine (JA-Ile) levels, while not affecting salicylic acid (SA) and indole-3-acetic acid (IAA) production. These findings shed new light on the modulation of plant immunity by Pi07586 and enhance our understanding of the intricate relationship between P. infestans and host plants.

【 授权许可】

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Copyright © 2023 Xiong, Zhao, Chen, Yuan, Zhang and Wang