Massively-multiplexed epitope mapping techniques for viral antigen discovery

Frontiers in Immunology

Immunology

Aaron T. Irving¹  Diya Hu² 

[1] Department of Clinical Laboratory Studies, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Centre for Infection, Immunity & Cancer, Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University, Haining, China;Biomedical and Health Translational Research Centre of Zhejiang Province (BIMET), Haining, China;College of Medicine & Veterinary Medicine, The University of Edinburgh, Edinburgh, United Kingdom;Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University, Haining, China;

关键词: epitope;    epitope mapping;    phage-display;    T cell;    B cell;   

DOI  :  10.3389/fimmu.2023.1192385

 received in 2023-03-23, accepted in 2023-09-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.

【 授权许可】

Unknown   
Copyright © 2023 Hu and Irving