Frontiers in Physiology | |
p90RSK2, a new MLCK mediates contractility in myosin light chain kinase null smooth muscle | |
Physiology | |
Jaspreet Kalra1  Zaneta Markowska1  Avril Somlyo1  Ramon J. Ayon1  Zygmunt S. Derewenda1  Aaron Franke2  Li Jin3  Hua Wang4  Mykhaylo Artamonov5  | |
[1] Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, United States;Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, United States;Brain Surgery Worldwide, Atlanta, GA, United States;Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, United States;Department of Orthopedics, University of Virginia, Charlottesville, VA, United States;Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, United States;Sentara Martha Jefferson Hospital, Charlottesville, VA, United States;Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, United States;Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States; | |
关键词: smooth muscle; MLCK; mylk1; p90 ribosomal S6 kinase; RSK2; PDK1; Pyk2; | |
DOI : 10.3389/fphys.2023.1228488 | |
received in 2023-05-24, accepted in 2023-08-24, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Introduction: Phosphorylation of smooth muscle (SM) myosin regulatory light chain (RLC20) is a critical switch leading to SM contraction. The canonical view held that only the short isoform of myosin light chain kinase (MLCK1) catalyzed this reaction. It is now accepted that auxiliary kinases may contribute to vascular SM tone and contractility. We have previously reported that p90 ribosomal S6 kinase (RSK2) functions as such a kinase, in parallel with MLCK1, contributing ∼25% of the maximal myogenic force in resistance arteries. Thus, RSK2 may be instrumental in the regulation of basal vascular tone and blood pressure. Here, we take advantage of a MLCK1 null mouse (mylk1−/−) to further test our hypothesis that RSK2 can function as an MLCK, playing a significant physiological role in SM contractility.Methods: Using fetal (E14.5-18.5) SM tissues, as embryos die at birth, we investigated the necessity of MLCK for contractility and fetal development and determined the ability of RSK2 kinase to compensate for the lack of MLCK and characterized its signaling pathway in SM.Results and Discussion: Agonists induced contraction and RLC20 phosphorylation in mylk1−/− SM was attenuated by RSK2 inhibition. The pCa-tension relationships in permeabilized strips of bladder showed no difference in Ca2+ sensitivity in WT vs mylk1−/− muscles, although the magnitude of force responses was considerably smaller in the absence of MLCK. The magnitude of contractile responses was similar upon addition of GTPγS to activate the RhoA/ROCK pathway or calyculinA to inhibit the myosin phosphatase. The Ca2+-dependent tyrosine kinase, Pyk2, contributed to RSK2-mediated contractility and RLC20 phosphorylation. Proximity-ligation and immunoprecipitation assays demonstrated an association of RSK2, PDK1 and ERK1/2 with MLCK and actin. RSK2, PDK1, ERK1/2 and MLCK formed a signaling complex on the actin filament, positioning them for interaction with adjacent myosin heads. The Ca2+-dependent component reflected the agonist mediated increases in Ca2+, which activated the Pyk2/PDK1/RSK2 signaling cascade. The Ca2+−independent component was through activation of Erk1/2/PDK1/RSK2 leading to direct phosphorylation of RLC20, to increase contraction. Overall, RSK2 signaling constitutes a new third signaling pathway, in addition to the established Ca2+/CaM/MLCK and RhoA/ROCK pathways to regulate SM contractility.
【 授权许可】
Unknown
Copyright © 2023 Kalra, Artamonov, Wang, Franke, Markowska, Jin, Derewenda, Ayon and Somlyo.
【 预 览 】
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RO202310122842538ZK.pdf | 2960KB | download |