Serum metabolomics analysis of biomarkers and metabolic pathways in patients with colorectal cancer associated with spleen-deficiency and qi-stagnation syndrome or damp-heat syndrome: a prospective cohort study

Frontiers in Oncology

Oncology

Hao Tu¹  Zeng-Qiang Yang²  Zi-Jie Yang³  Feng Gao³  Ya-Nan Wang³  Min Zou³  Ming-Bin Gui³  Ming Xu³  Wei-Qiang Wu³  Jin-Kai Feng⁴  Yan-Sheng Zhang⁵ 

[1] Department of Colorectal Surgery, Chongqing Qijiang District People’s Hospital, Chongqing, China;Department of Colorectal Surgery, Gansu Provincial Central Hospital, Lanzhou, China;Department of Colorectal and Anal Surgery, The 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, Lanzhou, China;Department of Hepatic Surgery VI, The Third Affiliated Hospital of Naval Medical University (Eastern Hepatobiliary Surgery Hospital), Shanghai, China;Department of Obstetrics and Gynecology, Gansu Provincial Maternity and Child-Care Hospital, Lanzhou, China;

关键词: colorectal cancer (CRC);    metabolomics;    liquid chromatography-mass spectrometry (LC-MS);    damp-heat syndrome (DHS);    spleen-deficiency and qi-stagnation syndrome (SDQSS);   

DOI  :  10.3389/fonc.2023.1190706

 received in 2023-04-04, accepted in 2023-08-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveTo profile the serum metabolites and metabolic pathways in colorectal cancer (CRC) patients associated with spleen-deficiency and qi-stagnation syndrome (SDQSS) or damp-heat syndrome (DHS).MethodsFrom May 2020 to January 2021, CRC patients diagnosed with traditional Chinese medicine (TCM) syndromes of SDQSS or DHS were enrolled. The clinicopathological data of the SDQSS and DHS groups were compared. The serum samples were analyzed by liquid chromatography-mass spectrometry (LC-MS). The variable importance in the projection >1, fold change ≥3 or ≤0.333, and P value ≤0.05 were used to identify differential metabolites between the two groups. Furthermore, areas under the receiver operating characteristic (ROC) curve > 0.9 were applied to select biomarkers with good predictive performance. The enrichment metabolic pathways were searched through the database of Kyoto Encyclopedia of Genes and Genomes.Results60 CRC patients were included (30 SDQSS and 30 DHS). The level of alanine aminotransferase was marginally significantly higher in the DHS group than the SDQSS group (P = 0.051). The other baseline clinicopathological characteristics were all comparable between the two groups. 23 differential serum metabolites were identified, among which 16 were significantly up-regulated and 7 were significantly down-regulated in the SDQSS group compared with the DHS group. ROC curve analysis showed that (S)-3-methyl-2-oxopentanoic acid, neocembrene, 1-aminocyclopropanecarboxylic acid, 3-methyl-3-hydroxypentanedioate, and nicotine were symbolic differential metabolites with higher predictive power. The top five enrichment signalling pathways were valine, leucine and isoleucine biosynthesis; lysosome; nicotine addiction; fructose and mannose metabolism; and pertussis.ConclusionOur study identifies the differential metabolites and characteristic metabolic pathways among CRC patients with SDQSS or DHS, offering the possibility of accurate and objective syndrome differentiation and TCM treatment for CRC patients.

【 授权许可】

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Copyright © 2023 Zou, Zhang, Feng, Tu, Gui, Wang, Yang, Yang, Xu, Wu and Gao