Fumigant activity and transcriptomic analysis of two plant essential oils against the tea green leafhopper, Empoasca onukii Matsuda

Frontiers in Physiology

Physiology

Can Li¹  Jifeng Hu²  Weiwen Tan³  Jinqiu Wang³  Tianyi Pu³  Ni Zhang³  Yuehua Song³ 

[1] Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guiyang University, Guiyang, China;Huaxi District Plant Protection Station of Guiyang City, Guiyang, China;School of Karst Science, Guizhou Normal University, Guiyang, China;State Engineering Technology Institute for Karst Desertification Control, Guiyang, China;

关键词: Empoasca onukii;    plant essential oil;    fumigant activity;    transcriptome;    detoxification genes;   

DOI  :  10.3389/fphys.2023.1217608

 received in 2023-05-05, accepted in 2023-09-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: The tea green leafhopper, Empoasca (Matsumurasca) onukii Matsuda, R., 1952 (Hemiptera: Cicadellidae), is currently one of the most devastating pests in the Chinese tea industry. The long-term use of chemical pesticides has a negative impact on human health, impeding the healthy and sustainable development of the tea industry in this region. Therefore, there is a need for non-chemical insecticides to control E. onukii in tea plants. The essential oils from plants have been identified for their potential insecticidal ability; however, there is a lack of knowledge regarding the effect of plant essential oils on E. onukii and its gene expression.Methods: In order to address these knowledge gaps, the components of Pogostemon cablin and Cinnamomum camphora essential oils were analyzed in the present study using gas chromatography‐mass spectrometry. The fumigation toxicity of two essential oils on E. onukii was tested using sealed conical flasks. In addition, We performed comparative transcriptome analyses of E. onukii treated with or without P. cablin essential oil.Results: The 36-h lethal concentration (LC50) values for E. onukii treated with P. cablin and C. camphora essential oils were 0.474 and 1.204 μL mL−1 respectively. Both essential oils exhibited the potential to control E. onukii, but the fumigation activity of P. cablin essential oil was more effective. A total of 2,309 differentially expressed genes were obtained by transcriptome sequencing of E. onukii treated with P. cablin essential oil.Conclusion: Many of differentially expressed genes were found to contain detoxifification genes, indicating that these families may have played an important role when E. onukii was exposed to essential oil stress. We also found differential expression of genes related to redox-related gene families, suggesting the upregulation of genes associated with possible development of drug and stress resistance. This work offers new insights for the prevention and management of E. onukii in the future.

【 授权许可】

Unknown   
Copyright © 2023 Tan, Zhang, Wang, Pu, Hu, Li and Song.