Substitution Mapping and Allelic Variations of the Domestication Genes from O. rufipogon and O. nivara

Rice

Research

Ping He¹  Haitao Zhu²  Xuelin Fu²  Ziqiang Liu²  Zhe Zhang²  Zisheng Guo²  Jianan Zhang²  Ruifeng Song²  Guiquan Zhang²  Tuo Zou²  Zhangqiang Wang² 

[1] College of Life Sciences, South China Agricultural University, 510642, Guangzhou, China;Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, 510642, Guangzhou, China;

关键词: Single segment substitution lines;    Domestication gene;    Substitution mapping;    Allelic variation;    O. rufipogon;    O. nivara;   

DOI  :  10.1186/s12284-023-00655-y

 received in 2023-04-14, accepted in 2023-08-15,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDomestication from wild rice species to cultivated rice is a key milestone, which involved changes of many specific traits and the variations of the genetic systems. Among the AA-genome wild rice species, O. rufipogon and O. nivara, have many favorable genes and thought to be progenitors of O. sativa.ResultsIn the present study, by using O. rufipogon and O. nivara as donors, the single segment substitution lines (SSSLs) have been developed in the background of the elite indica cultivar, HJX74. In the SSSLs population, 11 genes for 5 domestication traits, including tiller angle, spreading panicle, awn, seed shattering, and red pericarp, were identified and mapped on 5 chromosomes through substitution mapping. Herein, allelic variations of 7 genes were found through sequence alignment with the known genes, that is, TA7-RUF was allelic to PROG1, TA8-RUF was allelic to TIG1, SPR4-NIV was allelic to OsLG1, AN4-RUF was allelic to An-1, SH4-NIV was allelic to SH4, and both RC7-RUF and RC7-NIV were allelic to Rc. Meanwhile, 4 genes, TA11-NIV, SPR3-NIV, AN3-NIV, and AN4-NIV, were considered as the novel genes identified in these SSSLs, because of none known genes for the related domestication traits found in the chromosomal locations of them.ConclusionThe results indicated that the SSSLs would be precious germplasm resources for gene mining and utilization from wild rice species, and it laid the foundation for further analyses of the novel domestication genes to better understand the genetic basis in regulating the traits variation during domestication.

【 授权许可】

CC BY   
© Springer Science+Business Media, LLC, part of Springer Nature 2023

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