Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of the skeletal muscle

Skeletal Muscle

Research

Xiyue Chen¹  Feng Yue¹  Stephanie N. Oprescu²  Nick Baumann²  Shihuan Kuang³  Huating Wang⁴  Yu Zhao⁴  Qiang Sun⁴ 

[1]Department of Animal Sciences, Purdue University, 47907, West Lafayette, IN, USA

[2]Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA

[3]Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA

[4]Department of Animal Sciences, Purdue University, 47907, West Lafayette, IN, USA

[5]Center for Cancer Research, Purdue University, 47907, West Lafayette, IN, USA

[6]Department of Orthopedics and Traumatology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong

[7] Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong, China

关键词: Aging;    Differentiation;    Satellite cells;    Single-cell RNA-sequencing (scRNA-seq);    SRY-box transcription factor;    Stem cells;   

DOI  :  10.1186/s13395-023-00324-0

 received in 2023-03-09, accepted in 2023-08-24,  发布年份 2023

来源: Springer

【 摘 要 】

Transcription factors (TFs) play key roles in regulating differentiation and function of stem cells, including muscle satellite cells (MuSCs), a resident stem cell population responsible for postnatal regeneration of the skeletal muscle. Sox11 belongs to the Sry-related HMG-box (SOX) family of TFs that play diverse roles in stem cell behavior and tissue specification. Analysis of single-cell RNA-sequencing (scRNA-seq) datasets identify a specific enrichment of Sox11 mRNA in differentiating but not quiescent MuSCs. Consistent with the scRNA-seq data, Sox11 levels increase during differentiation of murine primary myoblasts in vitro. scRNA-seq data comparing muscle regeneration in young and old mice further demonstrate that Sox11 expression is reduced in aged MuSCs. Age-related decline of Sox11 expression is associated with reduced chromatin contacts within the topologically associating domains. Unexpectedly, Myod1Cre-driven deletion of Sox11 in embryonic myoblasts has no effects on muscle development and growth, resulting in apparently healthy muscles that regenerate normally. Pax7CreER- or Rosa26CreER- driven (MuSC-specific or global) deletion of Sox11 in adult mice similarly has no effects on MuSC differentiation or muscle regeneration. These results identify Sox11 as a novel myogenic differentiation marker with reduced expression in quiescent and aged MuSCs, but the specific function of Sox11 in myogenesis remains to be elucidated.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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