Sterilized Anopheles funestus can autodisseminate sufficient pyriproxyfen to the breeding habitat under semi-field settings

Malaria Journal

Research

Naomi Urio¹  Ali Ali¹  Halfan Ngowo¹  Mohamed Jumanne¹  Amos J. Ngonzi¹  Yohana A. Mwalugelo²  Augustino Mmbaga³  Dickson Lwetoijera³  Felista S. Tarimo³  Fredros Okumu⁴  Hamisi J. Kunambi⁵  Joseph Swilla⁶ 

[1] Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 53, Ifakara, Tanzania;Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 53, Ifakara, Tanzania;Department of Biomedical Sciences, Jaramogi Oginga Odinga University of Science and Technology, P.O. Box 210-40601, Bondo, Kenya;Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 53, Ifakara, Tanzania;School of Life Science and Bio-Engineering, The Nelson Mandela African Institution of Science and Technology, P.O. Box 447, Arusha, Tanzania;Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 53, Ifakara, Tanzania;School of Life Science and Bio-Engineering, The Nelson Mandela African Institution of Science and Technology, P.O. Box 447, Arusha, Tanzania;School of Public of Health, Faculty of Health Science, University of Witwatersrand, Johannesburg, South Africa;Institute of Biodiversity, Animal Health and, Comparative Medicine, University of Glasgow, G12 8QQ, Glasgow, UK;Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 53, Ifakara, Tanzania;School of Life Science and Bio-Engineering, The Nelson Mandela African Institution of Science and Technology, P.O. Box 447, Arusha, Tanzania;Tanzania Biotech Products Limited, The National Development Cooperation, P.O. Box 30119, Kibaha, Tanzania;School of Life Science and Bio-Engineering, The Nelson Mandela African Institution of Science and Technology, P.O. Box 447, Arusha, Tanzania;

关键词: Anopheles funestus;    Autodissemination;    Pyriproxyfen;    Sterilization;    Semi-field settings;   

DOI  :  10.1186/s12936-023-04699-9

 received in 2023-06-26, accepted in 2023-09-01,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAnopheles funestus, the main malaria vector, prefer to oviposit in permanent and/or semi-permanent breeding habitats located far from human dwellings. Difficulties in identifying and accessing these habitats jeopardize the feasibility of conventional larviciding. In this way, a semi-field study was conducted to assess the potential of autodissemination of pyriproxyfen (PPF) by An. funestus for its control.MethodsThe study was conducted inside a semi-field system (SFS). Therein, two identical separate chambers, the treatment chamber with a PPF-treated clay pot (0.25 g AI), and the control chamber with an untreated clay pot. In both chambers, one artificial breeding habitat made of a plastic basin with one litre of water was provided. Three hundred blood-fed female An. funestus aged 5–9 days were held inside untreated and treated clay pots for 30 min and 48 h before being released for oviposition. The impact of PPF on adult emergence, fecundity, and fertility through autodissemination and sterilization effects were assessed by comparing the treatment with its appropriate control group.ResultsMean (95% CI) percentage of adult emergence was 15.5% (14.9–16.1%) and 70.3% (69–71%) in the PPF and control chamber for females exposed for 30 min (p < 0.001); and 19% (12–28%) and 95% (88–98%) in the PPF and control chamber for females exposed for 48 h (p < 0.001) respectively. Eggs laid by exposed mosquitoes and their hatch rate were significantly reduced compared to unexposed mosquitoes (p < 0.001). Approximately, 90% of females exposed for 48 h retained abnormal ovarian follicles and only 42% in females exposed for 30 min.ConclusionThe study demonstrated sterilization and adult emergence inhibition via autodissemination of PPF by An. funestus. Also, it offers proof that sterilized An. funestus can transfer PPF to prevent adult emergence at breeding habitats. These findings warrant further assessment of the autodissemination of PPF in controlling wild population of An. funestus, and highlights its potential for complementing long-lasting insecticidal nets.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 5